Data provided by National Institute of Standards and Technology
This repository contains raw and intermediate data files as well as analysis code from the manuscript ?Evaluating the Analytical Performance of Direct-to-Consumer Gut Microbiome Testing Services?. In this analysis a pilot version of the homogenized whole human stool reference material was submitted as a sample to commercial direct to consumer microbiome testing companies. In addition, NIST conducted in house characterization by 16S amplicon sequencing and whole metagenomic sequencing (WMS) on the same sample. Raw sequencing data from the sequencing providers was not obtained for this study; however sequencing files (fastq) both 16S amplicon sequencing analysis and whole metagenomic sequencing (WMS) conducted at NIST are included. In addition, the fastq files from other donors that contributed to the pilot material and included in this analysis to represent biological diversity are also included in this record.
About this Dataset
Updated: 2025-04-06
Metadata Last Updated:
2024-06-05 00:00:00
Date Created:
N/A
Data Provided by:
Dataset Owner:
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| Title | Analytical Performance of Direct-to-Consumer Gut Microbiome Testing Services |
|---|---|
| Description | This repository contains raw and intermediate data files as well as analysis code from the manuscript ?Evaluating the Analytical Performance of Direct-to-Consumer Gut Microbiome Testing Services?. In this analysis a pilot version of the homogenized whole human stool reference material was submitted as a sample to commercial direct to consumer microbiome testing companies. In addition, NIST conducted in house characterization by 16S amplicon sequencing and whole metagenomic sequencing (WMS) on the same sample. Raw sequencing data from the sequencing providers was not obtained for this study; however sequencing files (fastq) both 16S amplicon sequencing analysis and whole metagenomic sequencing (WMS) conducted at NIST are included. In addition, the fastq files from other donors that contributed to the pilot material and included in this analysis to represent biological diversity are also included in this record. |
| Modified | 2024-06-05 00:00:00 |
| Publisher Name | National Institute of Standards and Technology |
| Contact | mailto:[email protected] |
| Keywords | Microbiome , Metagenomics , whole metagenomic sequencing , WMS , NGS , gut microbiome , 16S amplicon sequencing |
{
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"fn": "Stephanie Servetas"
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"landingPage": "https:\/\/data.nist.gov\/od\/id\/mds2-3348",
"title": "Analytical Performance of Direct-to-Consumer Gut Microbiome Testing Services",
"description": "This repository contains raw and intermediate data files as well as analysis code from the manuscript ?Evaluating the Analytical Performance of Direct-to-Consumer Gut Microbiome Testing Services?. In this analysis a pilot version of the homogenized whole human stool reference material was submitted as a sample to commercial direct to consumer microbiome testing companies. In addition, NIST conducted in house characterization by 16S amplicon sequencing and whole metagenomic sequencing (WMS) on the same sample. Raw sequencing data from the sequencing providers was not obtained for this study; however sequencing files (fastq) both 16S amplicon sequencing analysis and whole metagenomic sequencing (WMS) conducted at NIST are included. In addition, the fastq files from other donors that contributed to the pilot material and included in this analysis to represent biological diversity are also included in this record.",
"language": [
"en"
],
"distribution": [
{
"downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-3348\/README.txt",
"description": "Text file containing information\/descriptions of folders and files included in this repository",
"mediaType": "text\/plain",
"title": "README"
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{
"downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-3348\/publication_set.zip",
"format": "zip folder includes: fastq files (for 16S), r markdowns, rdata, csv, xlsx",
"description": "This folder contains code for two sets of data analyses (1) for NIST 16S amplicon sequencing and (2) for taxonomic profile comparisons in support of the evaluation of the analytical performance of DTC gut microbiome testing services conducted in R as well as input\/output data files. a complete description can be found in the README file",
"mediaType": "application\/zip",
"title": "publication_set"
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{
"downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-3348\/WMS_raw_reads.zip",
"format": "zip file contains fastq files within two folders depending on the donor",
"description": "The folder contains fastq files for data generated by NIST. a full description can be found in the README document",
"mediaType": "application\/zip",
"title": "WMS_raw_reads"
}
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"modified": "2024-06-05 00:00:00",
"publisher": {
"@type": "org:Organization",
"name": "National Institute of Standards and Technology"
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"theme": [
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"Health:Precision medicine"
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"keyword": [
"Microbiome",
"Metagenomics",
"whole metagenomic sequencing",
"WMS",
"NGS",
"gut microbiome",
"16S amplicon sequencing"
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}
