U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock () or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Breadcrumb

  1. Home

Construction of a Dual-enzyme, Subzero (-30 degree C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry

This tutorial provides mechanical drawings, electrical schematics, parts lists, STL files for 3D-printed parts, IGS files, and instructions for automated machining necessary for construction of a dual-protease, subzero, liquid chromatography system for hydrogen-deuterium exchange mass spectrometry. Electrical schematics for construction of a multi-zone temperature controller that regulate to ±0.1 oC are also included in this tutorial.

About this Dataset

Updated: 2024-02-22
Metadata Last Updated: 2019-02-20 00:00:00
Date Created: N/A
Views:
Data Provided by:
liquid chromatography
Dataset Owner: N/A

Access this data

Contact dataset owner Landing Page URL
Download URL
Table representation of structured data
Title Construction of a Dual-enzyme, Subzero (-30 degree C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry
Description This tutorial provides mechanical drawings, electrical schematics, parts lists, STL files for 3D-printed parts, IGS files, and instructions for automated machining necessary for construction of a dual-protease, subzero, liquid chromatography system for hydrogen-deuterium exchange mass spectrometry. Electrical schematics for construction of a multi-zone temperature controller that regulate to ±0.1 oC are also included in this tutorial.
Modified 2019-02-20 00:00:00
Publisher Name National Institute of Standards and Technology
Contact mailto:jeffrey.hudgens@nist.gov
Keywords liquid chromatography , hydrogen-deuterium exchange , mass spectrometry , precision , peptide , protein , proteolysis , proteomics , reference material , temperature control.
{
    "identifier": "ark:\/88434\/mds2-2151",
    "accessLevel": "public",
    "contactPoint": {
        "hasEmail": "mailto:jeffrey.hudgens@nist.gov",
        "fn": "Jeffrey W. Hudgens"
    },
    "programCode": [
        "006:045"
    ],
    "@type": "dcat:Dataset",
    "landingPage": "https:\/\/data.nist.gov\/od\/id\/mds2-2151",
    "description": "This tutorial provides  mechanical drawings, electrical schematics, parts lists, STL files for 3D-printed parts, IGS files, and instructions for automated machining necessary for construction of a dual-protease, subzero, liquid chromatography system for hydrogen-deuterium exchange mass spectrometry. Electrical schematics for construction of a multi-zone temperature controller that regulate to \u00b10.1 oC are also included in this tutorial.",
    "language": [
        "en"
    ],
    "title": "Construction of a Dual-enzyme, Subzero (-30 degree C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry",
    "distribution": [
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-2151\/T-controller%20%28ver%202%29.pdf",
            "mediaType": "application\/pdf"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-2151\/T-controller%20%28ver%202%29.pdf.sha256",
            "mediaType": "text\/plain"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-2151\/ADDENDUM%20for%20HDX.docx",
            "format": "word processing document",
            "description": "This addendum provides information for configuring the instrument with an 8-port valve in the Valve #2 location. The 8-port valve offers the ability to backflush the protease, trap, and analytical columns, which allows more rapid removal of protease aggregates from the top of each column.",
            "mediaType": "application\/vnd.openxmlformats-officedocument.wordprocessingml.document",
            "title": "ADDENDUM for HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 oC"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-2151\/Multi-Channel%20T-controller.pdf",
            "description": "This file presents mechanical drawings, electrical schematics, parts lists, STL file inventory for 3D-printed parts, and instructions for construction of the Multi-channel Temperature Controller",
            "mediaType": "application\/pdf",
            "title": "Construction information for the Multi-channel Temperature Controller"
        },
        {
            "accessURL": "https:\/\/doi.org\/10.18434\/M32151",
            "title": "DOI Access for Construction of a Dual-enzyme, Subzero (-30 degree C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-2151\/HDX-MS%20Analytical%20Instrument.pdf",
            "description": "This file presents mechanical drawings, electrical schematics, parts lists, lists of STL files for 3D-printed parts, lists of IGS files for automated machining, and instructions for construction of the HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 degree C",
            "mediaType": "application\/pdf",
            "title": "HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 degree C"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-2151\/Multi-Channel%20Valve%20Controller.pdf",
            "description": "This file presents mechanical drawings, electrical schematics, parts lists, STL file inventory for 3D-printed parts, and instructions for construction of the Multi-Channel Valve Controller.",
            "mediaType": "application\/pdf",
            "title": "Multi-Channel Valve Controller"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-2151\/Multi-Channel%20Valve%20Controller%20STL%20files.7z",
            "description": "This file contains the STL files for the 3D-printed parts used to construct the Multi-Channel Valve Controller",
            "mediaType": "application\/octet-stream",
            "title": "Multi-Channel Valve Controller STL files"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-2151\/ADDENDUM%20for%20HDX.docx.sha256",
            "mediaType": "text\/plain",
            "title": "SHA256 File for ADDENDUM for HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 oC"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-2151\/Multi-Channel%20T-controller.pdf.sha256",
            "mediaType": "text\/plain",
            "title": "SHA256 File for Construction information for the Multi-channel Temperature Controller"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-2151\/HDX-MS%20Analytical%20Instrument.pdf.sha256",
            "mediaType": "text\/plain",
            "title": "SHA256 File for HDX-MS Analytical Instrument for Incorporation on a Robotic Rail, Featuring Dual Enzyme Chambers and HPLC Separations at -30 degree C"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-2151\/Multi-Channel%20Valve%20Controller.pdf.sha256",
            "mediaType": "text\/plain",
            "title": "SHA256 File for Multi-Channel Valve Controller"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-2151\/Multi-Channel%20Valve%20Controller%20STL%20files.7z.sha256",
            "mediaType": "text\/plain",
            "title": "SHA256 File for Multi-Channel Valve Controller STL files"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-2151\/Multi-Channel%20T-controller%20STL%20Files.7z.sha256",
            "mediaType": "text\/plain",
            "title": "SHA256 File for STL Files for parts used to construct the Multi-channel Temperature Controller"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-2151\/HDX-MS%20Analytical%20Instrument%20STL-IGS%20Files.7z.sha256",
            "mediaType": "text\/plain",
            "title": "SHA256 File for STL and IGS files that accompany the file, HDX-MS Analytical Instrument.pdf"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-2151\/Multi-Channel%20T-controller%20STL%20Files.7z",
            "format": "7Z",
            "description": "STL Files for parts used to construct the Multi-channel Temperature Controller",
            "mediaType": "application\/octet-stream",
            "title": "STL Files for parts used to construct the Multi-channel Temperature Controller"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-2151\/HDX-MS%20Analytical%20Instrument%20STL-IGS%20Files.7z",
            "format": "7Z",
            "description": "This is the compressed file containing the STL and IGS files that accompany the file, HDX-MS Analytical Instrument.pdf.",
            "mediaType": "application\/octet-stream",
            "title": "STL and IGS files that accompany the file, HDX-MS Analytical Instrument.pdf"
        }
    ],
    "license": "https:\/\/www.nist.gov\/open\/license",
    "bureauCode": [
        "006:55"
    ],
    "modified": "2019-02-20 00:00:00",
    "publisher": {
        "@type": "org:Organization",
        "name": "National Institute of Standards and Technology"
    },
    "theme": [
        "Bioscience:Bioprocessing",
        "Chemistry:Analytical chemistry",
        "Physics:Biological physics",
        "Bioscience:Proteomics",
        "Metrology:Amount of substance"
    ],
    "issued": "2020-04-14",
    "keyword": [
        "liquid chromatography",
        "hydrogen-deuterium exchange",
        "mass spectrometry",
        "precision",
        "peptide",
        "protein",
        "proteolysis",
        "proteomics",
        "reference material",
        "temperature control."
    ]
}

Was this page helpful?