Data provided by National Oceanic and Atmospheric Administration
This dataset contains biological, chemical, optical, physical, and survey - biological data collected on R/V New Horizon during cruise NH1418 in the North Pacific Ocean and South Pacific Ocean from 2014-09-20 to 2014-10-06. These data include Nitrate, PAR, chlorophyll a, density, depth, dissolved Oxygen, fluorescence, nitrate plus nitrite, particulate organic Carbon (POC), particulate organic nitrogen, prochlorococcus abundance, salinity calculated from CTD primary sensors, and water temperature. The instruments used to collect these data include Automated Cell Counter, CHN Elemental Analyzer, CTD Sea-Bird SBE 911plus, Flow Cytometer, Niskin bottle, Turner Designs Fluorometer 10-AU, and WETLabs ECO-FLNTU. These data were collected by Michael W. Lomas of Bigelow Laboratory for Ocean Sciences and Adam Martiny of University of California-Irvine as part of the "Biological Controls on the Ocean C:N:P ratios (Biological C:N:P ratios)" project and "Dimensions of Biodiversity (Dimensions of Biodiversity)" and "Ocean Carbon and Biogeochemistry (OCB)" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-11-20.
The following is the text of the dataset description provided by BCO-DMO:
NH1418 ctd
Dataset Description:
Acquisition Description:
Temperature, salinity, oxygen concentration and saturation, and PAR were measured using a Sea-Bird SBE-911+ CTD platform equipped on the rosette deployment system. Fluorescence was measured via the rosette system using a WetLabs ECO AFL/FL platform.
Samples for NO3-/NO2- and NO2- were gravity filtered through 0.8 µm Nucleopore polycarbonate filters using acid cleaned in-line polycarbonate filter holders, then frozen (-20oC) in HDPE bottles until analysis on an Alpkem Flow Solution IV (Dore et al. 1996).
Soluble reactive phosphorus was measured after preparation via the magnesium-induced coprecipitation method (Karl and Tien 1992; Lomas et al. 2010).
Particulate organic carbon (POC), nitrogen (PON), and phosphorus samples were filtered on precombusted Whatman GF/F filters and frozen until analysis. After thawing, POC/PON filters were allowed to dry overnight at 65◦C before being packed into a 30 mm tin capsule (CE Elantech, Lakewood, New Jersey). Samples were then analyzed for C and N content on a FlashEA 1112 nitrogen and carbon analyzer (Thermo Scientific, Waltham, Massachusetts). POC and PON concentrations were calibrated using known quantities of atropine. Particulate organic phosphorus samples (POP) are analyzed using a ash-hydrolysis method (Lomas et al., 2010)
For chlorophyll, ~ 250–500 mL seawater was filtered onto 25-mm Ahlstrom glass fiber filters (nominal pore size 0.7 μm) under low pressure (15 kpa), and frozen immediately at −80_C. Samples were extracted in 90% acetone in the dark for 14–18 h at −20_C and quantified on a Turner 10-AU fluorometer using the acidification method (Parsons et al. 1984).
For cell counts, samples of whole seawater were collected in 2-mL centrifuge tubes, fixed with freshly made 0.2 -μm-filtered paraformaldehyde (0.5% v/v final concentration) for 1 h at 5_C in the dark, and counted on a FACSJazz or Influx flow cytometer (BD, Franklin Lakes, NJ, U.S.A.) utilizing a 200 mW 488 nm laser, with detectors for forward scatter, side scatter, 530 nm, and 692 nm. Prochlorococcus populations were discriminated based on forward scatter and red fluorescence, and a gate in orange (585 nm) discriminated for Synechococcus. Picoeukaryotic phytoplankton were all the red auto fluorescing cells that did not fit the Cyanobacteria gating scheme with a cell size below 2 – 3 μm.
See https://www.rvdata.us/search/cruise/NH1418 for further details.
For published methodologies please see the Related Publications section.
About this Dataset
Updated: 2024-02-22
Metadata Last Updated:
2023-05-14T06:47:14.876Z
Date Created:
N/A
Data Provided by:
Dataset Owner:
N/A
| Title | Depth profile data from R/V New Horizons NH1418 in the tropical Pacific from Sept-Oct. 2014 (NCEI Accession 0278177) |
|---|---|
| Description | This dataset contains biological, chemical, optical, physical, and survey - biological data collected on R/V New Horizon during cruise NH1418 in the North Pacific Ocean and South Pacific Ocean from 2014-09-20 to 2014-10-06. These data include Nitrate, PAR, chlorophyll a, density, depth, dissolved Oxygen, fluorescence, nitrate plus nitrite, particulate organic Carbon (POC), particulate organic nitrogen, prochlorococcus abundance, salinity calculated from CTD primary sensors, and water temperature. The instruments used to collect these data include Automated Cell Counter, CHN Elemental Analyzer, CTD Sea-Bird SBE 911plus, Flow Cytometer, Niskin bottle, Turner Designs Fluorometer 10-AU, and WETLabs ECO-FLNTU. These data were collected by Michael W. Lomas of Bigelow Laboratory for Ocean Sciences and Adam Martiny of University of California-Irvine as part of the "Biological Controls on the Ocean C:N:P ratios (Biological C:N:P ratios)" project and "Dimensions of Biodiversity (Dimensions of Biodiversity)" and "Ocean Carbon and Biogeochemistry (OCB)" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-11-20. The following is the text of the dataset description provided by BCO-DMO: NH1418 ctd Dataset Description: Acquisition Description: Temperature, salinity, oxygen concentration and saturation, and PAR were measured using a Sea-Bird SBE-911+ CTD platform equipped on the rosette deployment system. Fluorescence was measured via the rosette system using a WetLabs ECO AFL/FL platform. Samples for NO3-/NO2- and NO2- were gravity filtered through 0.8 µm Nucleopore polycarbonate filters using acid cleaned in-line polycarbonate filter holders, then frozen (-20oC) in HDPE bottles until analysis on an Alpkem Flow Solution IV (Dore et al. 1996). Soluble reactive phosphorus was measured after preparation via the magnesium-induced coprecipitation method (Karl and Tien 1992; Lomas et al. 2010). Particulate organic carbon (POC), nitrogen (PON), and phosphorus samples were filtered on precombusted Whatman GF/F filters and frozen until analysis. After thawing, POC/PON filters were allowed to dry overnight at 65◦C before being packed into a 30 mm tin capsule (CE Elantech, Lakewood, New Jersey). Samples were then analyzed for C and N content on a FlashEA 1112 nitrogen and carbon analyzer (Thermo Scientific, Waltham, Massachusetts). POC and PON concentrations were calibrated using known quantities of atropine. Particulate organic phosphorus samples (POP) are analyzed using a ash-hydrolysis method (Lomas et al., 2010) For chlorophyll, ~ 250–500 mL seawater was filtered onto 25-mm Ahlstrom glass fiber filters (nominal pore size 0.7 μm) under low pressure (15 kpa), and frozen immediately at −80_C. Samples were extracted in 90% acetone in the dark for 14–18 h at −20_C and quantified on a Turner 10-AU fluorometer using the acidification method (Parsons et al. 1984). For cell counts, samples of whole seawater were collected in 2-mL centrifuge tubes, fixed with freshly made 0.2 -μm-filtered paraformaldehyde (0.5% v/v final concentration) for 1 h at 5_C in the dark, and counted on a FACSJazz or Influx flow cytometer (BD, Franklin Lakes, NJ, U.S.A.) utilizing a 200 mW 488 nm laser, with detectors for forward scatter, side scatter, 530 nm, and 692 nm. Prochlorococcus populations were discriminated based on forward scatter and red fluorescence, and a gate in orange (585 nm) discriminated for Synechococcus. Picoeukaryotic phytoplankton were all the red auto fluorescing cells that did not fit the Cyanobacteria gating scheme with a cell size below 2 – 3 μm. See https://www.rvdata.us/search/cruise/NH1418 for further details. For published methodologies please see the Related Publications section. |
| Modified | 2023-05-14T06:47:14.876Z |
| Publisher Name | N/A |
| Contact | N/A |
| Keywords | 0278177 , CHLOROPHYLL A , DEPTH - OBSERVATION , DISSOLVED OXYGEN , FLUORESCENCE , NITRATE , nitrate + nitrite content (concentration) , PARTICULATE ORGANIC CARBON , PARTICULATE ORGANIC NITROGEN (PON) , PHOTOSYNTHETIC ACTIVE RADIATION (PAR) , Prochlorococcus , SALINITY , species abundance , WATER DENSITY , WATER TEMPERATURE , bottle , CHN Analyzer , CTD , Flow Cytometer , fluorometer , biological , chemical , optical , physical , survey - biological , NEW HORIZON , Bigelow Laboratory for Ocean Sciences , University of California - Irvine , Biological and Chemical Oceanography Data Management Office , North Pacific Ocean , South Pacific Ocean , oceanography , BCO-DMO > Biological and Chemical Oceanography Data Management Office , BIGELOW > Bigelow Laboratory of Ocean Sciences , Dimensions of Biodiversity (Dimensions of Biodiversity) , Ocean Carbon and Biogeochemistry (OCB) , Biological Controls on the Ocean C:N:P ratios (Biological C:N:P ratios) , NH1418 , Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1045966 , Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1046001 , Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1046297 , Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1046368 , ISO_DateTime_UTC , Nitrate , PAR , SRP , bottle , cast , cell_concentration , chlorophyll a , cruise id , density , depth , dissolved Oxygen , fluorescence , latitude , longitude , nitrate plus nitrite , particulate organic Carbon (POC) , particulate organic nitrogen , particulate organic phosphorus , pico_euks , prochlorococcus abundance , salinity calculated from CTD primary sensors , sample identification , station , synechococcus abundance , water temperature , yrday_utc , EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA > CYANOBACTERIA (BLUE-GREEN ALGAE) , EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CHLOROPHYLL , EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NITRATE , EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NITRITE , EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NITROGEN , EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ORGANIC CARBON , EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > OXYGEN , EARTH SCIENCE > OCEANS > OCEAN OPTICS > FLUORESCENCE , EARTH SCIENCE > OCEANS > OCEAN OPTICS > PHOTOSYNTHETICALLY ACTIVE RADIATION , EARTH SCIENCE > OCEANS > OCEAN TEMPERATURE > WATER TEMPERATURE , EARTH SCIENCE > OCEANS > SALINITY/DENSITY > DENSITY , EARTH SCIENCE > OCEANS > SALINITY/DENSITY > SALINITY , CTD_Fluorescence , CTD_PAR , Cast , Chla , Cruise , DNA_Cast , DNA_Depth , DNA_ISO_DateTime_UTC , DNA_Niskin_Bottle , DNA_Sample , Density , Depth , ISO_DateTime_UTC , Latitude , Longitude , Nanoeukaryotes , Nanoeukaryotes_POC_cell , Nitrate_Nitrite , Nitrite , Oxygen_Concentration , Oxygen_Saturation , POC , PON , POP , Picoeukaryotes , Picoeukaryotes_POC_cell , Prochlorococcus , Prochlorococcus_POC_cell , SRP , Salinity , Station , Synechococcus , Synechococcus_POC_cell , Temperature , yrday_utc , Automated Cell Counter , CHN Elemental Analyzer , CTD Sea-Bird SBE 911plus , Flow Cytometer , Niskin bottle , Turner Designs Fluorometer 10-AU , WETLabs ECO-FLNTU , CHN ANALYZERS > Carbon, Hydrogen, Nitrogen Analyzers , CTD > Conductivity, Temperature, Depth , FLOW CYTOMETRY , FLUOROMETERS > FLUOROMETERS , FACSJazz: Fluorescence Activated Cell Sorter (BD Biosciences) , FlashEA 112 , Influx Flow Cytometer , Sea-Bird SBE-911+ , unknown (Niskin bottle) , unknown (Turner Designs Fluorometer 10-AU) , unknown (WETLabs ECO-FLNTU) , R/V New Horizon , Ships , NEW HORIZON (ICES code: 32NM) , OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN , OCEAN > PACIFIC OCEAN > SOUTH PACIFIC OCEAN , Hawaii , environment , oceans , biota |
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"title": "Depth profile data from R\/V New Horizons NH1418 in the tropical Pacific from Sept-Oct. 2014 (NCEI Accession 0278177)",
"description": "This dataset contains biological, chemical, optical, physical, and survey - biological data collected on R\/V New Horizon during cruise NH1418 in the North Pacific Ocean and South Pacific Ocean from 2014-09-20 to 2014-10-06. These data include Nitrate, PAR, chlorophyll a, density, depth, dissolved Oxygen, fluorescence, nitrate plus nitrite, particulate organic Carbon (POC), particulate organic nitrogen, prochlorococcus abundance, salinity calculated from CTD primary sensors, and water temperature. The instruments used to collect these data include Automated Cell Counter, CHN Elemental Analyzer, CTD Sea-Bird SBE 911plus, Flow Cytometer, Niskin bottle, Turner Designs Fluorometer 10-AU, and WETLabs ECO-FLNTU. These data were collected by Michael W. Lomas of Bigelow Laboratory for Ocean Sciences and Adam Martiny of University of California-Irvine as part of the \"Biological Controls on the Ocean C:N:P ratios (Biological C:N:P ratios)\" project and \"Dimensions of Biodiversity (Dimensions of Biodiversity)\" and \"Ocean Carbon and Biogeochemistry (OCB)\" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2020-11-20.\n\nThe following is the text of the dataset description provided by BCO-DMO:\n\nNH1418 ctd\n\nDataset Description:\nAcquisition Description:\nTemperature, salinity, oxygen concentration and saturation, and PAR were measured using a Sea-Bird SBE-911+ CTD platform equipped on the rosette deployment system. Fluorescence was measured via the rosette system using a WetLabs ECO AFL\/FL platform.\n\nSamples for NO3-\/NO2- and NO2- were gravity filtered through 0.8 \u00b5m Nucleopore polycarbonate filters using acid cleaned in-line polycarbonate filter holders, then frozen (-20oC) in HDPE bottles until analysis on an Alpkem Flow Solution IV (Dore et al. 1996).\n\nSoluble reactive phosphorus was measured after preparation via the magnesium-induced coprecipitation method (Karl and Tien 1992; Lomas et al. 2010).\n\nParticulate organic carbon (POC), nitrogen (PON), and phosphorus samples were filtered on precombusted Whatman GF\/F filters and frozen until analysis. After thawing, POC\/PON filters were allowed to dry overnight at 65\u25e6C before being packed into a 30 mm tin capsule (CE Elantech, Lakewood, New Jersey). Samples were then analyzed for C and N content on a FlashEA 1112 nitrogen and carbon analyzer (Thermo Scientific, Waltham, Massachusetts). POC and PON concentrations were calibrated using known quantities of atropine. Particulate organic phosphorus samples (POP) are analyzed using a ash-hydrolysis method (Lomas et al., 2010)\n\nFor chlorophyll, ~ 250\u2013500 mL seawater was filtered onto 25-mm Ahlstrom glass fiber filters (nominal pore size 0.7 \u03bcm) under low pressure (15 kpa), and frozen immediately at \u221280_C. Samples were extracted in 90% acetone in the dark for 14\u201318 h at \u221220_C and quantified on a Turner 10-AU fluorometer using the acidification method (Parsons et al. 1984).\n\nFor cell counts, samples of whole seawater were collected in 2-mL centrifuge tubes, fixed with freshly made 0.2 -\u03bcm-filtered paraformaldehyde (0.5% v\/v final concentration) for 1 h at 5_C in the dark, and counted on a FACSJazz or Influx flow cytometer (BD, Franklin Lakes, NJ, U.S.A.) utilizing a 200 mW 488 nm laser, with detectors for forward scatter, side scatter, 530 nm, and 692 nm. Prochlorococcus populations were discriminated based on forward scatter and red fluorescence, and a gate in orange (585 nm) discriminated for Synechococcus. Picoeukaryotic phytoplankton were all the red auto fluorescing cells that did not fit the Cyanobacteria gating scheme with a cell size below 2 \u2013 3 \u03bcm.\n\nSee https:\/\/www.rvdata.us\/search\/cruise\/NH1418 for further details.\n\nFor published methodologies please see the Related Publications section.",
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