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Dissolved inorganic nitrogen, chlorophyll-a, and primary production from bioassay experiments during the R/V Hugh R. Sharp cruise HRS1414 in the Mid and South-Atlantic Bight in August of 2014 (DANCE project) (NCEI Accession 0277350)

This dataset contains biological and chemical data collected on R/V Hugh R. Sharp during cruise HRS1414 from 2014-08-01 to 2014-08-31. These data include Ammonium, chlorophyll a, nitrate plus nitrite, and primary production. The instruments used to collect these data include Fluorometer, Mass Spectrometer, and Nutrient Autoanalyzer. These data were collected by Dr Margaret Mulholland and Peter N. Sedwick of Old Dominion University and Dr Raymond Najjar of Pennsylvania State University as part of the "Collaborative Research: Impacts of atmospheric nitrogen deposition on the biogeochemistry of oligotrophic coastal waters (DANCE)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23.

The following is the text of the dataset description provided by BCO-DMO:

Acquisition Description: Experimental seawater collection: For shipboard bioassay experiments, whole seawater and resident biota were collected from ~4 m depth whilst underway at ~5 knots, using a trace-metal clean towfish system (Sedwick et al., 2011). This seawater was used to fill two 60 L polyethylene carboys in parallel inside a shipboard trace-metal clean container laboratory, after passing through pre-cleaned 180 µm nylon screen to exclude larger organisms, then subsequently used to fill the experimental incubation bottles (see below). Three bioassay experiments were performed (Sedwick et al., 2018), for which seawater was collected during three separate deployments of the towfish system.

For bioassay experiment 1, seawater was collected on 1 August, 2014, between 16:55 and 17:35 local time, between 38.6053°N, 72.2534°W (start) and 38.5692°N, 72.2354°W (end). Single determinations of iron and macronutrient concentrations in seawater from the towfish that was filtered in-line through a 0.8/0.2 µm AcroPak Supor filter capsule (Pall) yielded the following results:

Dissolved iron (DFe): 0.55 nM (start), 0.43 nM (end)

Dissolved nitrate+nitrite (NO3+NO2): 0.07 µM (start) 0.08 µM (end)

Dissolved phosphate (PO4): 0.19 µM (start), 0.20 µM (end)

Dissolved ammonium (NH4): not determined

For bioassay experiment 2, seawater was collected on 4 August, 2014, between 11:10 and 12:10 local time, between 38.3800°N, 72.4743°W (start) and 38.3847°N, 72.4761°W (end). Single determinations of iron and macronutrient concentrations in seawater from the towfish that was filtered in-line through a 0.8/0.2 µm AcroPak Supor filter capsule (Pall) yielded the following results:

Dissolved iron (DFe): 0.33 nM (start), 0.32 nM (end)

Dissolved nitrate+nitrite (NO3+NO2): 0.07 µM (start) 0.07 µM (end)

Dissolved phosphate (PO4): 0.19 µM (start), 0.20 µM (end)

Dissolved ammonium (NH4): 0.01 µM (start), 0.01 µM (end)

For bioassay experiment 3, seawater was collected on 9 August, 2014, between 15:19 and 15:54 local time, between 35.5305°N, 72.2760°W (start) and 35.5165°N, 72.2703°W (end). Single determinations of iron and macronutrient concentrations in seawater from the towfish that was filtered in-line through a 0.8/0.2 µm AcroPak Supor filter capsule (Pall) yielded the following results:

Dissolved iron (DFe): 0.89 nM (start), 0.90 nM (end)

Dissolved nitrate+nitrite (NO3+NO2): 0.05 µM (start) 0.07 µM (end)

Dissolved phosphate (PO4): not determined

Dissolved ammonium (NH4): 0.03 µM (start), 0.01 µM (end)

Bioassay experiment protocols: The shipboard bioassay experimental protocols are described by Sedwick et al. (2018). For each experiment there were 6 different incubation treatments (control, iron, nitrate, nitrate+iron, nitrate+iron+phosphate, rainwater), with triplicate bottles for each treatment sampled at each of three timepoints. Each bottle was completely subsampled for measurements of nutrients (NO3+NO2, NH4), chlorophyll-a and primary productivity. For the initial (time = 0) measurements, the seawater that remained in the 60 L polyethylene carboys after filling the incubation bottles was transfered into a 20 L polyethylene carboy from which subsamples were taken for measurements of NO3+NO2 after filtration through 0.8 µm pore size AcroDisc Supor syringe filters (Pall), for chlorophyll-a after filtration on to combusted 0.7 µm pore size GF/F filters (Whatman), and for incubation with carbon-13 labeled bicarbonate for estimation of primary production. For initial (t = 0) NH4 concentrations, we use mean values measured in seawater sampled from the towfish outlet after in-line filtration (see above).

Analytical procedures:

DFe: Filtered seawater samples were acidified at-sea to pH ~1.8 with Fisher Optima grade ultrapure hydrochloric acid, and then stored at room temperature until post-cruise analysis at Old Dominion University. Dissolved iron was determined by flow injection analysis with colorimetric detection after in-line preconcentration on resin-immobilized 8-hydroxyquinoline (Sedwick et al., 2015), using a method modified from Measures et al. (1995). Analyses were performed on a volumetric basis, so concentrations are reported in units of nanomole liter-1 (nM). Analytical precision is estimated from multiple (separate-day) determinations of the SAFe seawater reference materials, which yield uncertainties (expressed as one relative standard deviation on the mean, or one sigma) of ~15% at the concentration level of SAFe S seawater (0.090 nM), and ~10% at the concentration level of SAFe D2 seawater (0.90 nM). The analytical limit of detection is estimated as the DFe concentration equivalent to a peak area that is three times the standard deviation on the zero-loading blank (manifold blank), which yields an estimated detection limit below 0.04 nM (Bowie et al., 2004). Blank contributions from the ammonium acetate sample buffer solution (added on-line during analysis) and hydrochloric acid (added after collection) are negligible.

NO3+NO2: Dissolved nitrate and nitrite was determined at sea using an Astoria Pacific nutrient autoanalyzer using standard colorimetric methods with an estimated detection limit of 0.14 µM (Parsons et al., 1984; Price and Harrison, 1987). In surface waters, nitrate and nitrite were determined using the same autoanalyzer equipped with a liquid waveguide capillary cell (World Precision Instruments) (Zhang, 2000) to achieve an estimated detection limit of 0.02 µM.

PO4: Dissolved phosphate was determined at sea using an Astoria Pacific nutrient autoanalyzer using standard colorimetric methods with an estimated detection limit of 0.03 µM (Parsons et al., 1984; Price and Harrison, 1987).

NH4: Dissolved ammonium was determined at sea using the manual orthophthaldialdehyde method (Holmes et al., 1999), with an estimated detection limit of 10 nM.

Chl-a: Chlorophyll-a was determined at sea using the non-acidification method with a Turner 10-AU fluorometer (Welschmeyer, 1994).

PP: Primary production was measured using carbon stable istopes (Mulholland et al., 2006).

Missing data identifiers: ND = not determined (single measurement) NR = not reported (contamination likely, only used for NH4 data)

About this Dataset

Updated: 2024-02-22
Metadata Last Updated: 2023-04-02T21:12:37.765Z
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Dataset Owner: N/A

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Title Dissolved inorganic nitrogen, chlorophyll-a, and primary production from bioassay experiments during the R/V Hugh R. Sharp cruise HRS1414 in the Mid and South-Atlantic Bight in August of 2014 (DANCE project) (NCEI Accession 0277350)
Description This dataset contains biological and chemical data collected on R/V Hugh R. Sharp during cruise HRS1414 from 2014-08-01 to 2014-08-31. These data include Ammonium, chlorophyll a, nitrate plus nitrite, and primary production. The instruments used to collect these data include Fluorometer, Mass Spectrometer, and Nutrient Autoanalyzer. These data were collected by Dr Margaret Mulholland and Peter N. Sedwick of Old Dominion University and Dr Raymond Najjar of Pennsylvania State University as part of the "Collaborative Research: Impacts of atmospheric nitrogen deposition on the biogeochemistry of oligotrophic coastal waters (DANCE)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23. The following is the text of the dataset description provided by BCO-DMO: Acquisition Description: Experimental seawater collection: For shipboard bioassay experiments, whole seawater and resident biota were collected from ~4 m depth whilst underway at ~5 knots, using a trace-metal clean towfish system (Sedwick et al., 2011). This seawater was used to fill two 60 L polyethylene carboys in parallel inside a shipboard trace-metal clean container laboratory, after passing through pre-cleaned 180 µm nylon screen to exclude larger organisms, then subsequently used to fill the experimental incubation bottles (see below). Three bioassay experiments were performed (Sedwick et al., 2018), for which seawater was collected during three separate deployments of the towfish system. For bioassay experiment 1, seawater was collected on 1 August, 2014, between 16:55 and 17:35 local time, between 38.6053°N, 72.2534°W (start) and 38.5692°N, 72.2354°W (end). Single determinations of iron and macronutrient concentrations in seawater from the towfish that was filtered in-line through a 0.8/0.2 µm AcroPak Supor filter capsule (Pall) yielded the following results: Dissolved iron (DFe): 0.55 nM (start), 0.43 nM (end) Dissolved nitrate+nitrite (NO3+NO2): 0.07 µM (start) 0.08 µM (end) Dissolved phosphate (PO4): 0.19 µM (start), 0.20 µM (end) Dissolved ammonium (NH4): not determined For bioassay experiment 2, seawater was collected on 4 August, 2014, between 11:10 and 12:10 local time, between 38.3800°N, 72.4743°W (start) and 38.3847°N, 72.4761°W (end). Single determinations of iron and macronutrient concentrations in seawater from the towfish that was filtered in-line through a 0.8/0.2 µm AcroPak Supor filter capsule (Pall) yielded the following results: Dissolved iron (DFe): 0.33 nM (start), 0.32 nM (end) Dissolved nitrate+nitrite (NO3+NO2): 0.07 µM (start) 0.07 µM (end) Dissolved phosphate (PO4): 0.19 µM (start), 0.20 µM (end) Dissolved ammonium (NH4): 0.01 µM (start), 0.01 µM (end) For bioassay experiment 3, seawater was collected on 9 August, 2014, between 15:19 and 15:54 local time, between 35.5305°N, 72.2760°W (start) and 35.5165°N, 72.2703°W (end). Single determinations of iron and macronutrient concentrations in seawater from the towfish that was filtered in-line through a 0.8/0.2 µm AcroPak Supor filter capsule (Pall) yielded the following results: Dissolved iron (DFe): 0.89 nM (start), 0.90 nM (end) Dissolved nitrate+nitrite (NO3+NO2): 0.05 µM (start) 0.07 µM (end) Dissolved phosphate (PO4): not determined Dissolved ammonium (NH4): 0.03 µM (start), 0.01 µM (end) Bioassay experiment protocols: The shipboard bioassay experimental protocols are described by Sedwick et al. (2018). For each experiment there were 6 different incubation treatments (control, iron, nitrate, nitrate+iron, nitrate+iron+phosphate, rainwater), with triplicate bottles for each treatment sampled at each of three timepoints. Each bottle was completely subsampled for measurements of nutrients (NO3+NO2, NH4), chlorophyll-a and primary productivity. For the initial (time = 0) measurements, the seawater that remained in the 60 L polyethylene carboys after filling the incubation bottles was transfered into a 20 L polyethylene carboy from which subsamples were taken for measurements of NO3+NO2 after filtration through 0.8 µm pore size AcroDisc Supor syringe filters (Pall), for chlorophyll-a after filtration on to combusted 0.7 µm pore size GF/F filters (Whatman), and for incubation with carbon-13 labeled bicarbonate for estimation of primary production. For initial (t = 0) NH4 concentrations, we use mean values measured in seawater sampled from the towfish outlet after in-line filtration (see above). Analytical procedures: DFe: Filtered seawater samples were acidified at-sea to pH ~1.8 with Fisher Optima grade ultrapure hydrochloric acid, and then stored at room temperature until post-cruise analysis at Old Dominion University. Dissolved iron was determined by flow injection analysis with colorimetric detection after in-line preconcentration on resin-immobilized 8-hydroxyquinoline (Sedwick et al., 2015), using a method modified from Measures et al. (1995). Analyses were performed on a volumetric basis, so concentrations are reported in units of nanomole liter-1 (nM). Analytical precision is estimated from multiple (separate-day) determinations of the SAFe seawater reference materials, which yield uncertainties (expressed as one relative standard deviation on the mean, or one sigma) of ~15% at the concentration level of SAFe S seawater (0.090 nM), and ~10% at the concentration level of SAFe D2 seawater (0.90 nM). The analytical limit of detection is estimated as the DFe concentration equivalent to a peak area that is three times the standard deviation on the zero-loading blank (manifold blank), which yields an estimated detection limit below 0.04 nM (Bowie et al., 2004). Blank contributions from the ammonium acetate sample buffer solution (added on-line during analysis) and hydrochloric acid (added after collection) are negligible. NO3+NO2: Dissolved nitrate and nitrite was determined at sea using an Astoria Pacific nutrient autoanalyzer using standard colorimetric methods with an estimated detection limit of 0.14 µM (Parsons et al., 1984; Price and Harrison, 1987). In surface waters, nitrate and nitrite were determined using the same autoanalyzer equipped with a liquid waveguide capillary cell (World Precision Instruments) (Zhang, 2000) to achieve an estimated detection limit of 0.02 µM. PO4: Dissolved phosphate was determined at sea using an Astoria Pacific nutrient autoanalyzer using standard colorimetric methods with an estimated detection limit of 0.03 µM (Parsons et al., 1984; Price and Harrison, 1987). NH4: Dissolved ammonium was determined at sea using the manual orthophthaldialdehyde method (Holmes et al., 1999), with an estimated detection limit of 10 nM. Chl-a: Chlorophyll-a was determined at sea using the non-acidification method with a Turner 10-AU fluorometer (Welschmeyer, 1994). PP: Primary production was measured using carbon stable istopes (Mulholland et al., 2006). Missing data identifiers: ND = not determined (single measurement) NR = not reported (contamination likely, only used for NH4 data)
Modified 2023-04-02T21:12:37.765Z
Publisher Name N/A
Contact N/A
Keywords 0277350 , 277350 , AMMONIUM (NH4) , CHLOROPHYLL A , nitrate + nitrite content (concentration) , PRIMARY PRODUCTIVITY , fluorometer , mass spectrometer , nutrient autoanalyzer , biological , chemical , Hugh R. Sharp , Old Dominion University , The Pennsylvania State University , Biological and Chemical Oceanography Data Management Office , oceanography , BCO-DMO > Biological and Chemical Oceanography Data Management Office , Collaborative Research: Impacts of atmospheric nitrogen deposition on the biogeochemistry of oligotrophic coastal waters (DANCE) , HRS1414 , Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1260454 , Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1260574 , Ammonium , chlorophyll a , experiment id , incubation time or duration , nitrate plus nitrite , primary production , treatment , EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PRIMARY PRODUCTION , EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > AMMONIA , EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CHLOROPHYLL , EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NITRATE , EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NITRITE , SD_Chl_a , SD_NH4 , SD_NO3_NO2 , SD_PP , experiment , mean_Chl_a , mean_NH4 , mean_NO3_NO2 , mean_PP , time , treatment , Fluorometer , Mass Spectrometer , Nutrient Autoanalyzer , AUTOANALYZER > AUTOANALYZER , FLUOROMETERS > FLUOROMETERS , MASS SPECTROMETERS > MASS SPECTROMETERS , Astoria Pacific nutrient autoanalyzer , Europa 20/20 isotope ratio mass spectrometer , Shimadzu RF1501 , Turner Designs 10-AU fluorometer , R/V Hugh R. Sharp , Ships , Hugh R. Sharp (ICES code: 33H5) , Offshore Mid-Atlantic Bight and northern South-Atlantic Bight , environment , oceans
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    "title": "Dissolved inorganic nitrogen, chlorophyll-a, and primary production from bioassay experiments during the R\/V Hugh R. Sharp cruise HRS1414 in the Mid and South-Atlantic Bight in August of 2014 (DANCE project) (NCEI Accession 0277350)",
    "description": "This dataset contains biological and chemical data collected on R\/V Hugh R. Sharp during cruise HRS1414  from 2014-08-01 to 2014-08-31. These data include Ammonium, chlorophyll a, nitrate plus nitrite, and primary production. The instruments used to collect these data include Fluorometer, Mass Spectrometer, and Nutrient Autoanalyzer. These data were collected by Dr Margaret Mulholland and Peter N. Sedwick of Old Dominion University and Dr Raymond Najjar of Pennsylvania State University as part of the \"Collaborative Research: Impacts of atmospheric nitrogen deposition on the biogeochemistry of oligotrophic coastal waters (DANCE)\" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23.\n\nThe following is the text of the dataset description provided by BCO-DMO:\n\nAcquisition Description: Experimental seawater collection: For shipboard bioassay experiments, whole seawater and resident biota were collected from ~4 m depth whilst underway at ~5 knots, using a trace-metal clean towfish system (Sedwick et al., 2011). This seawater was used to fill two 60 L polyethylene carboys in parallel inside a shipboard trace-metal clean container laboratory, after passing through pre-cleaned 180 \u00b5m nylon screen to exclude larger organisms, then subsequently used to fill the experimental incubation bottles (see below). Three bioassay experiments were performed (Sedwick et al., 2018), for which seawater was collected during three separate deployments of the towfish system.\n\nFor bioassay experiment 1, seawater was collected on 1 August, 2014, between 16:55 and 17:35 local time, between 38.6053\u00b0N, 72.2534\u00b0W (start) and 38.5692\u00b0N, 72.2354\u00b0W (end). Single determinations of iron and macronutrient concentrations in seawater from the towfish that was filtered in-line through a 0.8\/0.2 \u00b5m AcroPak Supor filter capsule (Pall) yielded the following results:\n\nDissolved iron (DFe): 0.55 nM (start), 0.43 nM (end)\n\nDissolved nitrate+nitrite (NO3+NO2): 0.07 \u00b5M (start) 0.08 \u00b5M (end)\n\nDissolved phosphate (PO4): 0.19 \u00b5M (start), 0.20 \u00b5M (end)\n\nDissolved ammonium (NH4): not determined\n\nFor bioassay experiment 2, seawater was collected on 4 August, 2014, between 11:10 and 12:10 local time, between 38.3800\u00b0N, 72.4743\u00b0W (start) and 38.3847\u00b0N, 72.4761\u00b0W (end). Single determinations of iron and macronutrient concentrations in seawater from the towfish that was filtered in-line through a 0.8\/0.2 \u00b5m AcroPak Supor filter capsule (Pall) yielded the following results:\n\nDissolved iron (DFe): 0.33 nM (start), 0.32 nM (end)\n\nDissolved nitrate+nitrite (NO3+NO2): 0.07 \u00b5M (start) 0.07 \u00b5M (end)\n\nDissolved phosphate (PO4): 0.19 \u00b5M (start), 0.20 \u00b5M (end)\n\nDissolved ammonium (NH4): 0.01 \u00b5M (start), 0.01 \u00b5M (end)\n\nFor bioassay experiment 3, seawater was collected on 9 August, 2014, between 15:19 and 15:54 local time, between 35.5305\u00b0N, 72.2760\u00b0W (start) and 35.5165\u00b0N, 72.2703\u00b0W (end). Single determinations of iron and macronutrient concentrations in seawater from the towfish that was filtered in-line through a 0.8\/0.2 \u00b5m AcroPak Supor filter capsule (Pall) yielded the following results:\n\nDissolved iron (DFe): 0.89 nM (start), 0.90 nM (end)\n\nDissolved nitrate+nitrite (NO3+NO2): 0.05 \u00b5M (start) 0.07 \u00b5M (end)\n\nDissolved phosphate (PO4): not determined\n\nDissolved ammonium (NH4): 0.03 \u00b5M (start), 0.01 \u00b5M (end)\n\nBioassay experiment protocols: The shipboard bioassay experimental protocols are described by Sedwick et al. (2018). For each experiment there were 6 different incubation treatments (control, iron, nitrate, nitrate+iron, nitrate+iron+phosphate, rainwater), with triplicate bottles for each treatment sampled at each of three timepoints. Each bottle was completely subsampled for measurements of nutrients (NO3+NO2, NH4), chlorophyll-a and primary productivity. For the initial (time = 0) measurements, the seawater that remained in the 60 L polyethylene carboys after filling the incubation bottles was transfered into a 20 L polyethylene carboy from which subsamples were taken for measurements of NO3+NO2 after filtration through 0.8 \u00b5m pore size AcroDisc Supor syringe filters (Pall), for chlorophyll-a after filtration on to combusted 0.7 \u00b5m pore size GF\/F filters (Whatman), and for incubation with carbon-13 labeled bicarbonate for estimation of primary production. For initial (t = 0) NH4 concentrations, we use mean values measured in seawater sampled from the towfish outlet after in-line filtration (see above).\n\nAnalytical procedures:\n\nDFe: Filtered seawater samples were acidified at-sea to pH ~1.8 with Fisher Optima grade ultrapure hydrochloric acid, and then stored at room temperature until post-cruise analysis at Old Dominion University. Dissolved iron was determined by flow injection analysis with colorimetric detection after in-line preconcentration on resin-immobilized 8-hydroxyquinoline (Sedwick et al., 2015), using a method modified from Measures et al. (1995). Analyses were performed on a volumetric basis, so concentrations are reported in units of nanomole liter-1 (nM). Analytical precision is estimated from multiple (separate-day) determinations of the SAFe seawater reference materials, which yield uncertainties (expressed as one relative standard deviation on the mean, or one sigma) of ~15% at the concentration level of SAFe S seawater (0.090 nM), and ~10% at the concentration level of SAFe D2 seawater (0.90 nM). The analytical limit of detection is estimated as the DFe concentration equivalent to a peak area that is three times the standard deviation on the zero-loading blank (manifold blank), which yields an estimated detection limit below 0.04 nM (Bowie et al., 2004). Blank contributions from the ammonium acetate sample buffer solution (added on-line during analysis) and hydrochloric acid (added after collection) are negligible.\n\nNO3+NO2: Dissolved nitrate and nitrite was determined at sea using an Astoria Pacific nutrient autoanalyzer using standard colorimetric methods with an estimated detection limit of 0.14 \u00b5M (Parsons et al., 1984; Price and Harrison, 1987). 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