Data provided by National Oceanic and Atmospheric Administration
Water bottle samples were collected from selected depths (variable with station) at 14 stations in the study area. Coulter counts in the 14-28 um size class were routinely determined for comparison with the microscope counts. Water samples were filtered through 40 um Nytex mesh and centrifuged for 10 minutes at 1200 x g to isolate Karenia brevis. Pellets were frozen for subsequent analysis (pg cell-1) of total lipid, neutral lipid, free amino acids, protein and RNA. The biochemical determinations were performed within two weeks of collection at Kamykowski's NCSU laboratory. Aliquots were passed through 0.4 um filters; the filters were placed in 90% acetone for subsequent analysis of chlorophyll and the supernatant was analyzed for nitrate.
About this Dataset
Updated: 2024-02-22
Metadata Last Updated:
2025-11-19T15:17:31.573Z
Date Created:
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Dataset Owner:
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| Title | ECOHAB: Kamykowski_D- Florida Process Cruise 1998-11-16 to 1998-11-19 Karenia brevis counts, biochemistry and behavior (NCEI Accession 0000534) |
|---|---|
| Description | Water bottle samples were collected from selected depths (variable with station) at 14 stations in the study area. Coulter counts in the 14-28 um size class were routinely determined for comparison with the microscope counts. Water samples were filtered through 40 um Nytex mesh and centrifuged for 10 minutes at 1200 x g to isolate Karenia brevis. Pellets were frozen for subsequent analysis (pg cell-1) of total lipid, neutral lipid, free amino acids, protein and RNA. The biochemical determinations were performed within two weeks of collection at Kamykowski's NCSU laboratory. Aliquots were passed through 0.4 um filters; the filters were placed in 90% acetone for subsequent analysis of chlorophyll and the supernatant was analyzed for nitrate. |
| Modified | 2025-11-19T15:17:31.573Z |
| Publisher Name | N/A |
| Contact | N/A |
| Keywords | 0000534 , BIOCHEMISTRY , SPECIES IDENTIFICATION - COUNT , bottle , biological , plankton , North Carolina State University , Florida Marine Research Institute , Ecology and Oceanography of Harmful Algal Blooms (ECOHAB) , Coastal Waters of Florida , oceanography , DOC/NOAA/NESDIS/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce , FL/FWC/FMRI > Florida Marine Research Institute, Fish & Wildlife Conservation Commission, Florida , ECOHAB > Ecology and Oceanography of Harmful Algal Bloom , EARTH SCIENCE > AGRICULTURE > ANIMAL SCIENCE > ANIMAL PHYSIOLOGY AND BIOCHEMISTRY , abundance , algae , aquatic , chemistry , distribution , dynamic , marine , organic , plankton , population , saltwater , statistics , OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN , Destin Beach area , Florida Panhandle , environment , oceans , biota |
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