Water bottle samples were collected from selected depths (variable with station) at 44 stations in the study area. Coulter counts in the 14 to 28 um size class were routinely determined for comparison with the microscope counts provided by other researchers on the cruise. At six stations, water samples were filtered through a 40 um Nytex mesh and centrifuged for 10 minutes at 1200 x g and then frozen to isolate Karenia brevis cells for subsequent analysis (pg cell-1) of cellular lipid, free amino acid, and protein. The biochemical determinations were performed within two weeks of collection at Kamykowski's laboratory at NCSU.
About this Dataset
| Title | ECOHAB: Kamykowski_D- Florida Process Cruise: Karenia brevis counts, biochemistry and behavior, 1998-11-16 to 1998-11-19 (NCEI Accession 0000533) |
|---|---|
| Description | Water bottle samples were collected from selected depths (variable with station) at 44 stations in the study area. Coulter counts in the 14 to 28 um size class were routinely determined for comparison with the microscope counts provided by other researchers on the cruise. At six stations, water samples were filtered through a 40 um Nytex mesh and centrifuged for 10 minutes at 1200 x g and then frozen to isolate Karenia brevis cells for subsequent analysis (pg cell-1) of cellular lipid, free amino acid, and protein. The biochemical determinations were performed within two weeks of collection at Kamykowski's laboratory at NCSU. |
| Modified | 2025-11-19T15:17:31.286Z |
| Publisher Name | N/A |
| Contact | N/A |
| Keywords | 0000533 , BIOCHEMISTRY , SPECIES IDENTIFICATION - COUNT , bottle , biological , plankton , North Carolina State University , Florida Marine Research Institute , Ecology and Oceanography of Harmful Algal Blooms (ECOHAB) , Coastal Waters of Florida , oceanography , DOC/NOAA/NESDIS/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce , FL/FWC/FMRI > Florida Marine Research Institute, Fish & Wildlife Conservation Commission, Florida , ECOHAB > Ecology and Oceanography of Harmful Algal Bloom , EARTH SCIENCE > AGRICULTURE > ANIMAL SCIENCE > ANIMAL PHYSIOLOGY AND BIOCHEMISTRY , abundance , algae , aquatic , chemistry , distribution , dynamic , marine , organic , plankton , population , saltwater , statistics , OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN , Charlotte Harbor , Florida , Gulf of Mexico , environment , oceans , biota |
{
"identifier": "gov.noaa.nodc:0000533",
"accessLevel": "public",
"contactPoint": {
"@type": "vcard:Contact",
"fn": "Your contact point",
"hasEmail": "mailto:[email protected]"
},
"programCode": [
"010:000"
],
"landingPage": "",
"title": "ECOHAB: Kamykowski_D- Florida Process Cruise: Karenia brevis counts, biochemistry and behavior, 1998-11-16 to 1998-11-19 (NCEI Accession 0000533)",
"description": "Water bottle samples were collected from selected depths (variable with station) at 44 stations in the study area. Coulter counts in the 14 to 28 um size class were routinely determined for comparison with the microscope counts provided by other researchers on the cruise. At six stations, water samples were filtered through a 40 um Nytex mesh and centrifuged for 10 minutes at 1200 x g and then frozen to isolate Karenia brevis cells for subsequent analysis (pg cell-1) of cellular lipid, free amino acid, and protein. The biochemical determinations were performed within two weeks of collection at Kamykowski's laboratory at NCSU.",
"language": "",
"distribution": [
{
"@type": "dcat:Distribution",
"mediaType": "application\/json",
"accessURL": "https:\/\/www.ncei.noaa.gov\/metadata\/geoportal\/\/rest\/metadata\/item\/gov.noaa.nodc%3A0000533"
},
{
"@type": "dcat:Distribution",
"mediaType": "text\/html",
"accessURL": "https:\/\/www.ncei.noaa.gov\/metadata\/geoportal\/\/rest\/metadata\/item\/gov.noaa.nodc%3A0000533\/html"
},
{
"@type": "dcat:Distribution",
"mediaType": "application\/xml",
"accessURL": "https:\/\/www.ncei.noaa.gov\/metadata\/geoportal\/\/rest\/metadata\/item\/gov.noaa.nodc%3A0000533\/xml"
},
{
"@type": "dcat:Distribution",
"mediaType": "application\/octet-stream",
"accessURL": "https:\/\/www.ncei.noaa.gov\/access\/metadata\/landing-page\/bin\/gfx?id=gov.noaa.nodc:0000533"
}
],
"bureauCode": [
"010:04"
],
"modified": "2025-11-19T15:17:31.286Z",
"publisher": {
"@type": "org:Organization",
"name": "Your Publisher"
},
"theme": "",
"keyword": [
"0000533",
"BIOCHEMISTRY",
"SPECIES IDENTIFICATION - COUNT",
"bottle",
"biological",
"plankton",
"North Carolina State University",
"Florida Marine Research Institute",
"Ecology and Oceanography of Harmful Algal Blooms (ECOHAB)",
"Coastal Waters of Florida",
"oceanography",
"DOC\/NOAA\/NESDIS\/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce",
"FL\/FWC\/FMRI > Florida Marine Research Institute, Fish & Wildlife Conservation Commission, Florida",
"ECOHAB > Ecology and Oceanography of Harmful Algal Bloom",
"EARTH SCIENCE > AGRICULTURE > ANIMAL SCIENCE > ANIMAL PHYSIOLOGY AND BIOCHEMISTRY",
"abundance",
"algae",
"aquatic",
"chemistry",
"distribution",
"dynamic",
"marine",
"organic",
"plankton",
"population",
"saltwater",
"statistics",
"OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN",
"Charlotte Harbor",
"Florida",
"Gulf of Mexico",
"environment",
"oceans",
"biota"
]
}