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Effects of ocean acidification on shell condition and survival of Puget Sound pteropods from laboratory experiment studies from 2012-05-10 to 2012-07-12 (NCEI Accession 0125008)

This dataset contains laboratory experiment data that were collected to examine the effects of ocean acidification on shell condition and survival of Puget Sound pteropods. We tested whether the thecosome pteropod Limacina helicina from Puget Sound, an urbanized estuary in the northwest continental US, experiences shell dissolution and altered mortality rates when exposed to the high CO2, low aragonite saturation state (Ωa) conditions that occur in Puget Sound and the northeast Pacific Ocean. Five, week-long experiments were conducted in which we incubated pteropods collected from Puget Sound in four carbon chemistry conditions: current summer surface (~460-500 μatm CO2, Ωa ≈ 1.59), current deep water or surface conditions during upwelling (~760 and ~1600-1700 μatm CO2, Ωa ≈ 1.17 and 0.56), and future deep water or surface conditions during upwelling (~2800-3400 μatm CO2, Ωa ≈ 0.28). We measured shell condition using a scoring regime of five shell characteristics that capture different aspects of shell dissolution. We characterized carbon chemistry conditions in statistical analyses with Ωa, and conducted analyses considering Ωa both as a continuous dataset and as discrete treatments. Shell dissolution increased linearly as aragonite saturation state decreased. Discrete treatment comparisons indicate that shell dissolution was greater in undersaturated treatments compared to oversaturated treatments. Survival increased linearly with aragonite saturation state, though discrete treatment comparisons indicated that survival was similar in all but the lowest saturation state treatment. These results indicate that, under starvation conditions, pteropod survival may not be greatly affected by current and expected near-future aragonite saturation state in the NE Pacific, but shell dissolution may. Given that subsurface waters in Puget Sound’s main basin are undersaturated with respect to aragonite in the winter and can be undersaturated in the summer, the condition and persistence of the species in this estuary warrants further study.

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Updated: 2024-02-22
Metadata Last Updated: 2025-11-19T15:42:19.603Z
Date Created: N/A
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Dataset Owner: N/A

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Title Effects of ocean acidification on shell condition and survival of Puget Sound pteropods from laboratory experiment studies from 2012-05-10 to 2012-07-12 (NCEI Accession 0125008)
Description This dataset contains laboratory experiment data that were collected to examine the effects of ocean acidification on shell condition and survival of Puget Sound pteropods. We tested whether the thecosome pteropod Limacina helicina from Puget Sound, an urbanized estuary in the northwest continental US, experiences shell dissolution and altered mortality rates when exposed to the high CO2, low aragonite saturation state (Ωa) conditions that occur in Puget Sound and the northeast Pacific Ocean. Five, week-long experiments were conducted in which we incubated pteropods collected from Puget Sound in four carbon chemistry conditions: current summer surface (~460-500 μatm CO2, Ωa ≈ 1.59), current deep water or surface conditions during upwelling (~760 and ~1600-1700 μatm CO2, Ωa ≈ 1.17 and 0.56), and future deep water or surface conditions during upwelling (~2800-3400 μatm CO2, Ωa ≈ 0.28). We measured shell condition using a scoring regime of five shell characteristics that capture different aspects of shell dissolution. We characterized carbon chemistry conditions in statistical analyses with Ωa, and conducted analyses considering Ωa both as a continuous dataset and as discrete treatments. Shell dissolution increased linearly as aragonite saturation state decreased. Discrete treatment comparisons indicate that shell dissolution was greater in undersaturated treatments compared to oversaturated treatments. Survival increased linearly with aragonite saturation state, though discrete treatment comparisons indicated that survival was similar in all but the lowest saturation state treatment. These results indicate that, under starvation conditions, pteropod survival may not be greatly affected by current and expected near-future aragonite saturation state in the NE Pacific, but shell dissolution may. Given that subsurface waters in Puget Sound’s main basin are undersaturated with respect to aragonite in the winter and can be undersaturated in the summer, the condition and persistence of the species in this estuary warrants further study.
Modified 2025-11-19T15:42:19.603Z
Publisher Name N/A
Contact N/A
Keywords 0125008 , DISSOLVED INORGANIC CARBON (DIC) , pH , SALINITY , total alkalinity , spectrophotometer , biological , laboratory experiments , NOAA Northwest Fisheries Science Center , NOAA Northwest Fisheries Science Center , NODC Ocean Acidification Scientific Data Stewardship (OADS) , US DOC; NOAA; Office of Oceanic and Atmospheric Research; Ocean Acidification Program (OAP) , Puget Sound , oceanography , DOC/NOAA/NESDIS/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce , DOC/NOAA/NMFS/NWFSC > Northwest Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce , Ocean Acidification Program (OAP) , Ocean Carbon and Acidification Data System (OCADS) Project , EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ALKALINITY , EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > INORGANIC CARBON , EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH , EARTH SCIENCE > OCEANS > SALINITY/DENSITY > SALINITY , Laboratory experiment , Limacina helicina , human eye , pteropods , shell condition , steroscope , survival rate , Arag , Cat1 , Cat2 , Cat3 , Cat4 , Cat5 , DIC , Dead , Durafet , Live , Ratio , Salinity , Spec , Sum , TA , pCO2 , pH , OPTSPEC > Optical Spectrometer , OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN , Port Susan, Puget Sound, Washington , West coast of the U.S. , environment , oceans
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