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High-volume, label-free imaging for quantifying single-cell dynamics in iPSC colonies

This collection is comprised of multiple sets of experiments involving iPSCs with varying dosages of fluorescent light excitation. Samples were imaged every 2 minutes using phase-contrast microscopy for 20+ hours with or without fluorescence excitation. Each experiment was done on different days (i.e. exp0 vs exp1) but with triplicate wells (i.e. exp1-0, exp1-1, exp1-2). Additionally, data used to train our 2D U-Net and 3D U-Net models are included. Detailed information for each dataset can be found in Dataset_Information.xlsx.

About this Dataset

Updated: 2024-02-22
Metadata Last Updated: 2023-03-17 00:00:00
Date Created: N/A
Views:
Data Provided by:
genetic engineering; regenerative medicine
Dataset Owner: N/A

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Table representation of structured data
Title High-volume, label-free imaging for quantifying single-cell dynamics in iPSC colonies
Description This collection is comprised of multiple sets of experiments involving iPSCs with varying dosages of fluorescent light excitation. Samples were imaged every 2 minutes using phase-contrast microscopy for 20+ hours with or without fluorescence excitation. Each experiment was done on different days (i.e. exp0 vs exp1) but with triplicate wells (i.e. exp1-0, exp1-1, exp1-2). Additionally, data used to train our 2D U-Net and 3D U-Net models are included. Detailed information for each dataset can be found in Dataset_Information.xlsx.
Modified 2023-03-17 00:00:00
Publisher Name National Institute of Standards and Technology
Contact mailto:[email protected]
Keywords genetic engineering; regenerative medicine , pluripotent cell lines; quantitative live cell imaging; fluorescence microscopy; image data analysis; CNN , AI , U-Net
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}

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