Data provided by National Institute of Standards and Technology
The data presents the genome sequencing and assembly of Escherichia coli NIST0056. This study utilized E. coli as a model organism to introduce a highly efficient DNA extraction protocol that employs Adaptive Focused Acoustics® (AFA) technology. This method achieves an optimal balance between cell lysis and DNA integrity. Additionally, it takes genome copy numbers into consideration to estimate DNA extraction efficiency. With E. coli, this protocol has achieved nearly 100% cell lysis, establishing a new benchmark for performance. This innovative approach allows for accurate measurement of DNA quantities, facilitating techniques such as metagenomics and the development of new DNA extraction methods. Overall, this novel method addresses a longstanding limitation in microbiological research and has the potential to significantly enhance accuracy and reproducibility across a wide range of applications.
About this Dataset
Updated: 2026-09-19
Metadata Last Updated:
2025-06-17 00:00:00
Date Created:
N/A
Data Provided by:
Dataset Owner:
N/A
| Title | The integration of focused ultrasonication, ddPCR, and flow cytometry effectively estimates genome copies per cell and enhances DNA extraction efficiency. |
|---|---|
| Description | The data presents the genome sequencing and assembly of Escherichia coli NIST0056. This study utilized E. coli as a model organism to introduce a highly efficient DNA extraction protocol that employs Adaptive Focused Acoustics® (AFA) technology. This method achieves an optimal balance between cell lysis and DNA integrity. Additionally, it takes genome copy numbers into consideration to estimate DNA extraction efficiency. With E. coli, this protocol has achieved nearly 100% cell lysis, establishing a new benchmark for performance. This innovative approach allows for accurate measurement of DNA quantities, facilitating techniques such as metagenomics and the development of new DNA extraction methods. Overall, this novel method addresses a longstanding limitation in microbiological research and has the potential to significantly enhance accuracy and reproducibility across a wide range of applications. |
| Modified | 2025-06-17 00:00:00 |
| Publisher Name | National Institute of Standards and Technology |
| Contact | mailto:[email protected] |
| Keywords | Escherichia coli NIST0056 , DNA sequencing and genome assembly |
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