{
    "identifier": "gov.noaa.nodc:0123400",
    "accessLevel": "public",
    "contactPoint": {
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        "fn": "Your contact point",
        "hasEmail": "mailto:[email protected]"
    },
    "programCode": [
        "010:000"
    ],
    "landingPage": "",
    "title": "The effects of ocean acidification on hemocyte of crab species in Alaska from laboratory experiment studies from 2011-07-01 to 2013-07-06 (NCEI Accession 0123400)",
    "description": "Flow cytometry provides a rapid and reproducible method for analyzing crustacean hemocytes and their functions under experimentally-varied environmental conditions.  We used flow cytometry to determine if there was a difference in hematology and selected immune functions, and intracellular pH (pHi), under two different, future ocean acidification scenarios (pH = 7.51, 7.80) compared to current conditions (pH = 8.06) for Chionoecetes bairdi, the Tanner crab. Hemocytes were analyzed after adult Tanner crabs were held for two years under continuous exposure to acidified ocean water.  Total counts of hemocytes did not vary among experimental control and treatments; however, there was a significantly greater number of dead, circulating hemocytes in crabs held at the lowest pH treatment.  Phagocytosis of fluorescent microbeads by hemocytes was greatest at the lowest pH treatment.  These results suggest that hemocytes were dying, likely by apoptosis, at a rate faster than upregulated phagocytosis was able to remove moribund cells from circulation at the lowest pH.  There was no significant difference in pHi within hyalinocytes among pH levels, with apparent regulation to a mean pHi of 7.24, significantly lower than the external environment.  In contrast, there was a significant difference between treatments in pHi of the semi-granular+granular cells. These findings suggest that future, predicted levels of ocean acidification may affect the defense cells of Tanner crabs, possibly making them more susceptible to other stressors in the environment.",
    "language": "",
    "distribution": [
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            "accessURL": "https:\/\/www.ncei.noaa.gov\/access\/metadata\/landing-page\/bin\/gfx?id=gov.noaa.nodc:0123400"
        }
    ],
    "bureauCode": [
        "010:04"
    ],
    "modified": "2025-11-19T15:41:44.234Z",
    "publisher": {
        "@type": "org:Organization",
        "name": "Your Publisher"
    },
    "theme": "",
    "keyword": [
        "0123400",
        "biological data",
        "CELL COUNTS",
        "CRUSTACEANS",
        "MICROBIOLOGY",
        "pH",
        "SHELLFISH",
        "Flow Cytometer",
        "biological",
        "laboratory analyses",
        "NOAA Alaska Fisheries Science Center",
        "US DOC\/NOAA\/NMFS\/Northeast Fisheries Science Center\/Milford Laboratory",
        "NODC Ocean Acidification Scientific Data Stewardship (OADS)",
        "Ocean Carbon and Acidification Data System (OCADS)",
        "US DOC; NOAA; Office of Oceanic and Atmospheric Research; Ocean Acidification Program (OAP)",
        "Gulf of Alaska",
        "North Pacific Ocean",
        "oceanography",
        "DOC\/NOAA\/NESDIS\/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce",
        "DOC\/NOAA\/NMFS\/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce",
        "DOC\/NOAA\/NMFS\/NEFSC > Northeast Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce",
        "Ocean Acidification Program (OAP)",
        "Ocean Carbon and Acidification Data System (OCADS) Project",
        "EARTH SCIENCE > BIOLOGICAL CLASSIFICATION",
        "EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS\/INVERTEBRATES > ARTHROPODS > CRUSTACEANS",
        "EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS\/INVERTEBRATES > MOLLUSKS",
        "EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH",
        "Laboratory experiment",
        "external hemolymph pH",
        "hemocytes",
        "internal cell pH",
        "intracellular pH",
        "% Dead Cell SGC+GC",
        "% Dead HC",
        "% Phagocytosis",
        "Average pH",
        "Calculated HC pHi",
        "Calculated SGC+GC pHi",
        "Dead Cell HC",
        "Dead Cell SGC+GC",
        "Hemolymph pHe",
        "Raw data SNARF-5F HC FL2-H to calculate internal cell pH",
        "Raw data SNARF-5F SGC+GC FL2-H to calculate internal cell pH",
        "Raw data SNARF-5F SNARF-5F HC FL3-H used to calculate internal pH",
        "Raw data SNARF-5F SNARF-5F SGC+GC FL3-H used to calculate internal pH",
        "Total Cell HC",
        "Total Cell SGC+GC",
        "FLOW CYTOMETRY",
        "OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN",
        "OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN > GULF OF ALASKA",
        "Gulf of Alaska",
        "North Pacific Ocean",
        "environment",
        "oceans",
        "biota"
    ]
}