{
    "identifier": "gov.noaa.nodc:0225335",
    "accessLevel": "public",
    "contactPoint": {
        "@type": "vcard:Contact",
        "fn": "Your contact point",
        "hasEmail": "mailto:[email protected]"
    },
    "programCode": [
        "010:000"
    ],
    "landingPage": "",
    "title": "Black sea bass, Centropristis striata, first 48 hour response to increased levels of carbon dioxide from 2013-07-13 to 2015-07-10 (NCEI Accession 0225335)",
    "description": "The effects of elevated levels of CO2 on black sea bass, especially during the early life stages when fish tend to be more sensitive to OA effects, was investigated.  In this study, we exposed fertilized eggs to a range of CO2 levels (182.7 \u03bcatm- 2252.6 \u03bcatm) and measured % hatch, unhatch, and skeletal abnormalities after 48 hours of exposure. Adult male and female black sea bass were held in flowing seawater at ambient temperatures during the winters of 2012-2013, 2013-2014, and 2014-2015.  Once fish came out of torpor, adults were fed squid during conditioning and spawning. Gamete development in fish occurred naturally and spawning took place in holding tanks in late July of all three years. Fertilized eggs were collected in screens placed at the seawater outflow and exposed to different levels of CO2.",
    "language": "",
    "distribution": [
        {
            "@type": "dcat:Distribution",
            "mediaType": "application\/json",
            "accessURL": "https:\/\/www.ncei.noaa.gov\/metadata\/geoportal\/\/rest\/metadata\/item\/gov.noaa.nodc%3A0225335"
        },
        {
            "@type": "dcat:Distribution",
            "mediaType": "text\/html",
            "accessURL": "https:\/\/www.ncei.noaa.gov\/metadata\/geoportal\/\/rest\/metadata\/item\/gov.noaa.nodc%3A0225335\/html"
        },
        {
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            "mediaType": "application\/xml",
            "accessURL": "https:\/\/www.ncei.noaa.gov\/metadata\/geoportal\/\/rest\/metadata\/item\/gov.noaa.nodc%3A0225335\/xml"
        },
        {
            "@type": "dcat:Distribution",
            "mediaType": "application\/octet-stream",
            "accessURL": "https:\/\/www.ncei.noaa.gov\/access\/metadata\/landing-page\/bin\/gfx?id=gov.noaa.nodc:0225335"
        }
    ],
    "bureauCode": [
        "010:04"
    ],
    "modified": "2025-11-19T16:18:17.872Z",
    "publisher": {
        "@type": "org:Organization",
        "name": "Your Publisher"
    },
    "theme": "",
    "keyword": [
        "0225335",
        "CALCITE CONCENTRATION",
        "DISSOLVED INORGANIC CARBON (DIC)",
        "partial pressure of carbon dioxide - water",
        "pH",
        "SALINITY",
        "microscope",
        "multi-parameter water quality sensor",
        "Non-dispersive infrared (NDIR) dissolved inorganic carbon (DIC) analyzer",
        "spectrophotometer",
        "chemical",
        "laboratory experiments",
        "physical",
        "NOAA Northeast Fisheries Science Center",
        "US DOC\/NOAA\/NMFS\/Northeast Fisheries Science Center\/Milford Laboratory",
        "US DOC\/NOAA\/NMFS\/Northeast Fisheries Science Center\/Milford Laboratory",
        "NODC Ocean Acidification Scientific Data Stewardship (OADS)",
        "Ocean Carbon and Acidification Data System (OCADS)",
        "US DOC; NOAA; Office of Oceanic and Atmospheric Research; Ocean Acidification Program (OAP)",
        "Long Island Sound",
        "North Atlantic Ocean",
        "oceanography",
        "DOC\/NOAA\/NMFS\/NEFSC > Northeast Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce",
        "Ocean Acidification Program (OAP)",
        "Ocean Carbon and Acidification Data System (OCADS) Project",
        "EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY",
        "EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE",
        "EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > INORGANIC CARBON",
        "EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH",
        "EARTH SCIENCE > OCEANS > SALINITY\/DENSITY > SALINITY",
        "Laboratory experiment",
        "% not hatched",
        "Abnormal",
        "Calcite",
        "DIC",
        "Date",
        "Hatch",
        "Salinity",
        "pCO2 out",
        "pH",
        "CO2NDIR > Carbon Dioxide Nondispersive Infrared Analyzer",
        "MICROSCOPES > MICROSCOPES",
        "OPTSPEC > Optical Spectrometer",
        "OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN",
        "OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > LONG ISLAND SOUND",
        "Long Island Sound Connecticut Water",
        "environment",
        "oceans"
    ]
}