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Variability and Bias in Microbiome Metagenomic Sequencing: an Interlaboratory Study Comparing Experimental Protocols

This repository provides analysis code and results produced during evaluation of metagenomic sequencing (MGS) data collected through the Mosaic Standards Challenge. The Mosaic Standards Challenge asked participating laboratories analyze the same set of 7 samples using their own favored MGS laboratory methods. Each lab submitted their raw sequencing results and protocol information. The resulting MGS data was analyzed through a common bioinformatic pipeline and then evaluated to determine the effects of methodological choices.

About this Dataset

Updated: 2025-04-06
Metadata Last Updated: 2023-02-03 00:00:00
Date Created: N/A
Data Provided by:
Dataset Owner: N/A

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Table representation of structured data
Title Variability and Bias in Microbiome Metagenomic Sequencing: an Interlaboratory Study Comparing Experimental Protocols
Description This repository provides analysis code and results produced during evaluation of metagenomic sequencing (MGS) data collected through the Mosaic Standards Challenge. The Mosaic Standards Challenge asked participating laboratories analyze the same set of 7 samples using their own favored MGS laboratory methods. Each lab submitted their raw sequencing results and protocol information. The resulting MGS data was analyzed through a common bioinformatic pipeline and then evaluated to determine the effects of methodological choices.
Modified 2023-02-03 00:00:00
Publisher Name National Institute of Standards and Technology
Contact mailto:[email protected]
Keywords microbiome; methodology; metagenomic sequencing
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    "title": "Variability and Bias in Microbiome Metagenomic Sequencing: an Interlaboratory Study Comparing Experimental Protocols",
    "description": "This repository provides analysis code and results produced during evaluation of metagenomic sequencing (MGS) data collected through the Mosaic Standards Challenge. The Mosaic Standards Challenge asked participating laboratories analyze the same set of 7 samples using their own favored MGS laboratory methods. Each lab submitted their raw sequencing results and protocol information. The resulting MGS data was analyzed through a common bioinformatic pipeline and then evaluated to determine the effects of methodological choices. ",
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    "theme": [
        "Bioscience:Microbial measurements"
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    "keyword": [
        "microbiome; methodology; metagenomic sequencing"
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}