This dataset contains chemical and physical data collected on R/V Atlantis during cruise AT26-10 from 2014-01-03 to 2014-01-20. These data include Ammonium, Nitrate, pH, water pressure, and water temperature. The instruments used to collect these data include Isobaric Gas-Tight Sampler, Microscope-Electron, Microscope-Optical, and Shipboard Incubator. These data were collected by Dr Dionysis Foustoukos of Carnegie Institution for Science as part of the "An Integrated Study of Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents (Microbial Communities at Deep-Sea Vents)" project and "Center for Dark Energy Biosphere Investigations (C-DEBI)" and "Dimensions of Biodiversity (Dimensions of Biodiversity)" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2017-02-13.
The following is the text of the dataset description provided by BCO-DMO:
Results of on-board incubations of microbes in diffuse flow vent fluids collected from Crab Spa and Alvinella patch
Dataset Description:
This dataset includes results from shipboard high-pressure incubations of diffuse flow vent fluids collected from the Crab Spa (9.8398º N, 104.2913º W) and Alvinella (9.8398º N, 104.2915º W) sites at East Pacific Rise during the AT26-10 oceanographic expedition in January 2014. Reported parameters include dates and time elapsed, flow rate, temperature, pressure, and pH, and concentrations of NO3, NH4, H2, H2S, CH4.
Vent fluids used in onboard incubations were corrected from diffuse flow vent sites at the East Pacific Rise (2503 m): Crab Spa (9.8398º N, 104.2913º W) and Alvinella (9.8398º N, 104.2915º W) (see description in McNichol et al. [2016]). Fluids were collected using isobaric gas-tight samplers [Seewald et al., 2002] prior to their transfer to the shipboard continuous culture system [Foustoukos and Perez-Rodriguez, 2015]. Here, high-pressure incubations (250 bars) were conducted at mesophilic (30 ºC) and thermophilic (50 ºC) conditions to constrain the function and metabolic rates of denitrifying and DNRA microbial communities residing at Crab Spa and Alvinella, respectively. To enhance the activity of nitrate-respiring anaerobic bacteria, an NO3- (5 mm) and H2(aq) (1.30 mM)-enriched medium was introduced in the high-pressure incubations under strictly anaerobic conditions. Dissolved HCO3- (7.3 mm, 13C labeled) was used as added carbon source. Vent fluids were introduced at a flow rate of 0.042 mL/min, while growth medium was added at a rate of 0.0042 mL/min. The two sets of experiments took place for 356 (Crab Spa) and 50 hours (Alvinella). Direct cell counts were conducted by staining cells with 0.1% acridine orange and counting them with a fluorescence microscope. 15N/14N isotopic analysis of the NO3-, NH4+ and biomass were conducted with a Thermo Scientific Delta VPlus mass spectrometer and CE Instruments NA 2500 series elemental analyzer (EA).
References:
Foustoukos, D., and I. Perez-Rodriguez (2015), A continuous culture system for assessing microbial activities in the piezosphere, Applied and Environmental Microbiology, 81(19), 6850-6856.
McNichol, J., S. P. Sylva, F. Thomas, C. D. Taylor, S. M. Sievert, and J. S. Seewald (2016), Assessing microbial processes in deep-sea hydrothermal systems by incubation at in situ temperature and pressure, Deep Sea Research Part I: Oceanographic Research Papers, 115, 221-232.
Seewald, J. S., K. W. Doherty, T. R. Hammar, and S. P. Liberatore (2002), A new gas-tight isobaric sampler for hydrothermal fluids, Deep-Sea Research, Part I: Oceanographic Research Papers, 49(1), 189-196.
About this Dataset
| Title | Results from shipboard high-pressure incubations of diffuse flow vent fluids collected from the Crab Spa and Alvinella sites at East Pacific Rise during the AT26-10 expedition, Jan. 2014 (Microbial Communities at Deep-Sea Vents project) (NCEI Accession 0277894) |
|---|---|
| Description | This dataset contains chemical and physical data collected on R/V Atlantis during cruise AT26-10 from 2014-01-03 to 2014-01-20. These data include Ammonium, Nitrate, pH, water pressure, and water temperature. The instruments used to collect these data include Isobaric Gas-Tight Sampler, Microscope-Electron, Microscope-Optical, and Shipboard Incubator. These data were collected by Dr Dionysis Foustoukos of Carnegie Institution for Science as part of the "An Integrated Study of Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents (Microbial Communities at Deep-Sea Vents)" project and "Center for Dark Energy Biosphere Investigations (C-DEBI)" and "Dimensions of Biodiversity (Dimensions of Biodiversity)" programs. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2017-02-13. The following is the text of the dataset description provided by BCO-DMO: Results of on-board incubations of microbes in diffuse flow vent fluids collected from Crab Spa and Alvinella patch Dataset Description: This dataset includes results from shipboard high-pressure incubations of diffuse flow vent fluids collected from the Crab Spa (9.8398º N, 104.2913º W) and Alvinella (9.8398º N, 104.2915º W) sites at East Pacific Rise during the AT26-10 oceanographic expedition in January 2014. Reported parameters include dates and time elapsed, flow rate, temperature, pressure, and pH, and concentrations of NO3, NH4, H2, H2S, CH4. Vent fluids used in onboard incubations were corrected from diffuse flow vent sites at the East Pacific Rise (2503 m): Crab Spa (9.8398º N, 104.2913º W) and Alvinella (9.8398º N, 104.2915º W) (see description in McNichol et al. [2016]). Fluids were collected using isobaric gas-tight samplers [Seewald et al., 2002] prior to their transfer to the shipboard continuous culture system [Foustoukos and Perez-Rodriguez, 2015]. Here, high-pressure incubations (250 bars) were conducted at mesophilic (30 ºC) and thermophilic (50 ºC) conditions to constrain the function and metabolic rates of denitrifying and DNRA microbial communities residing at Crab Spa and Alvinella, respectively. To enhance the activity of nitrate-respiring anaerobic bacteria, an NO3- (5 mm) and H2(aq) (1.30 mM)-enriched medium was introduced in the high-pressure incubations under strictly anaerobic conditions. Dissolved HCO3- (7.3 mm, 13C labeled) was used as added carbon source. Vent fluids were introduced at a flow rate of 0.042 mL/min, while growth medium was added at a rate of 0.0042 mL/min. The two sets of experiments took place for 356 (Crab Spa) and 50 hours (Alvinella). Direct cell counts were conducted by staining cells with 0.1% acridine orange and counting them with a fluorescence microscope. 15N/14N isotopic analysis of the NO3-, NH4+ and biomass were conducted with a Thermo Scientific Delta VPlus mass spectrometer and CE Instruments NA 2500 series elemental analyzer (EA). References: Foustoukos, D., and I. Perez-Rodriguez (2015), A continuous culture system for assessing microbial activities in the piezosphere, Applied and Environmental Microbiology, 81(19), 6850-6856. McNichol, J., S. P. Sylva, F. Thomas, C. D. Taylor, S. M. Sievert, and J. S. Seewald (2016), Assessing microbial processes in deep-sea hydrothermal systems by incubation at in situ temperature and pressure, Deep Sea Research Part I: Oceanographic Research Papers, 115, 221-232. Seewald, J. S., K. W. Doherty, T. R. Hammar, and S. P. Liberatore (2002), A new gas-tight isobaric sampler for hydrothermal fluids, Deep-Sea Research, Part I: Oceanographic Research Papers, 49(1), 189-196. |
| Modified | 2023-05-05T11:47:50.395Z |
| Publisher Name | N/A |
| Contact | N/A |
| Keywords | 0277894 , 277894 , AMMONIUM (NH4) , HYDROSTATIC PRESSURE , NITRATE , pH , WATER TEMPERATURE , microscope , chemical , physical , R/V Atlantis , Biological and Chemical Oceanography Data Management Office , oceanography , BCO-DMO > Biological and Chemical Oceanography Data Management Office , Center for Dark Energy Biosphere Investigations (C-DEBI) , Dimensions of Biodiversity (Dimensions of Biodiversity) , An Integrated Study of Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents (Microbial Communities at Deep-Sea Vents) , AT26-10 , Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1136608 , Ammonium , Nitrate , date end , date_start , experiment id , no standard parameter , pH , sulfide , time_elapsed , water pressure , water temperature , Concentration of ammonium {NH4} per unit volume of the water body [unknown phase] by colorimetric autoanalysis , Elapsed time , Pressure (spatial co-ordinate) exerted by the water body by profiling pressure sensor and corrected to read zero at sea level , Temperature (ITS-90) of the water body , pH per unit volume of the water body , EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > AMMONIA , EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NITRATE , EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH , EARTH SCIENCE > OCEANS > OCEAN PRESSURE > WATER PRESSURE , EARTH SCIENCE > OCEANS > OCEAN TEMPERATURE > WATER TEMPERATURE , Ammonium , CH4 , H2 , NO3 , date_end , date_start , exp_id , flow_rate , pH , pressure , sulfide , temperature , time_elapsed , Isobaric Gas-Tight Sampler , Microscope-Electron , Microscope-Optical , Shipboard Incubator , MICROSCOPES > MICROSCOPES , IGT Sampler , custom high pressure bioreactor , unknown (Microscope-Electron) , unknown (Microscope-Optical) , R/V Atlantis , Ships , ATLANTIS (ICES code: 33AT) , East Pacific Rise, Pacific Ocean , environment , oceans |
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"title": "Results from shipboard high-pressure incubations of diffuse flow vent fluids collected from the Crab Spa and Alvinella sites at East Pacific Rise during the AT26-10 expedition, Jan. 2014 (Microbial Communities at Deep-Sea Vents project) (NCEI Accession 0277894)",
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