Flow cytometry provides a rapid and reproducible method for analyzing crustacean hemocytes and their functions under experimentally-varied environmental conditions. We used flow cytometry to determine if there was a difference in hematology and selected immune functions, and intracellular pH (pHi), under two different, future ocean acidification scenarios (pH = 7.51, 7.80) compared to current conditions (pH = 8.06) for Chionoecetes bairdi, the Tanner crab. Hemocytes were analyzed after adult Tanner crabs were held for two years under continuous exposure to acidified ocean water.

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This dataset contains the discrete bottle (CTD profile) data of the first dedicated West Coast Ocean Acidification cruise (WCOA2011). The cruise took place August 12-30, 2011 aboard the R/V Wecoma. Ninety-five stations were occupied from northern Washington to southern California along thirteen transect lines. At all stations, CTD casts were conducted, and discrete water samples were collected in Niskin bottles. The cruise was designed to obtain a synoptic snapshot of key carbon, physical, and biogeochemical parameters as they relate to ocean acidification (OA) in the coastal realm.

This cruise represents the second dedicated West Coast Ocean Acidification cruise (WCOA2012), which took place September 4-17, 2012 aboard NOAA Ship Bell M. Shimada. Seventy-seven stations were occupied from northern Washington to southern California along fourteen transect lines. At all stations, CTD casts were conducted, and discrete water samples were collected in Niskin bottles. The cruise was designed to obtain a synoptic snapshot of key carbon, physical, and biogeochemical parameters as they relate to ocean acidification (OA) in the coastal realm.

Observational oceanographic data obtained by an autonomous moored CTD profiler, thermistor mooring, acoustic Doppler current profiler, acoustic Doppler velocimeter, and benthic frame-mounted optical sensors, from the southern shelf of Monterey Bay, California, USA, 2011-2012.

This dataset contains the surface underway pCO2 data of the first dedicated West Coast Ocean Acidification cruise (WCOA2011). The cruise took place August 12-30, 2011 aboard the R/V Wecoma. Ninety-five stations were occupied from northern Washington to southern California along thirteen transect lines. At all stations, CTD casts were conducted, and discrete water samples were collected in Niskin bottles. Underway measurements of pCO2 were collected during the duration of the cruise.

The ALOHA Cabled Observatory (ACO) is a system of hardware and software that extends electric power and the Internet offshore, supporting sustained real-time observations in the deep ocean. The ACO is connected to Oahu, Hawaii by the HAW-4 telecommunications cable transferred to the project by AT&T in 2007. On June 6th, 2011, the ACO was deployed on the ocean bottom (depth ~ 5 kilometers - 3 miles) near Station ALOHA, 100 kilometers (60 nautical miles) north of Oahu, Hawaii.

Spur and groove (SAG) formations are found on the forereefs of many coral reefs worldwide. Modeling results have shown that SAG formations together with shoaling waves induce a nearshore Lagrangian circulation pattern of counter-rotating circulation cells, but these have never been observed in the field. We present results from two separate field studies of SAG formations on Palmyra Atoll which show their effect on waves to be small, but reveal a persistent order 1 cm/s depth-averaged Lagrangian offshore flow over the spur and onshore flow over the grooves, among other conclusions.

This dataset was collected as part of the Chukchi Sea Environmental Studies Program (CSESP), a multi-year, interdisciplinary ecological study focused on areas in outer continental shelf oil and gas leases in the northeastern Chukchi Sea. In 2008 when the program began, two 30 x 30 nautical mile study area boxes were developed around areas of interest to ConocoPhillips and Shell. The ConocoPhillips study area (named Klondike), and the Shell study area (named Burger) became the focus of data collection. Data were collected from these two study areas again in 2009.

This dataset contains laboratory experiment data that were collected to examine the effects of ocean acidification on hatch size and larval growth of walleye pollock (Theragra chalcogramma). Rising atmospheric concentrations of CO2 are predicted to decrease the pH of high-latitude oceans by 0.3-0.5 units by 2100. Because of their limited capacity for ion exchange, embryos and larvae of marine fishes are predicted to be more sensitive to elevated CO2 than juveniles and adults.