{
    "identifier": "ark:\/88434\/mds2-3092",
    "accessLevel": "public",
    "contactPoint": {
        "hasEmail": "mailto:[email protected]",
        "fn": "Samuel P. Forry"
    },
    "programCode": [
        "006:045"
    ],
    "@type": "dcat:Dataset",
    "landingPage": "https:\/\/data.nist.gov\/od\/id\/mds2-3092",
    "description": "This repository provides the raw data, analysis code, and results generated during a systematic evaluation of the impact of selected experimental protocol choices on the metagenomic sequencing analysis of microbiome samples. Briefly, a full factorial experimental design was implemented varying biological sample (n=5), operator (n=2), lot (n=2), extraction kit (n=2), 16S variable region (n=2), and reference database (n=3), and the main effects were calculated and compared between parameters (bias effects) and samples (real biological differences). A full description of the effort is provided in the associated publication.",
    "language": [
        "en"
    ],
    "title": "A Sensitivity Analysis of Methodological Variables Associated with Microbiome Measurements",
    "distribution": [
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            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-3092\/00README.rtf",
            "mediaType": "application\/rtf",
            "title": "00README"
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            "mediaType": "text\/plain",
            "title": "dada2_output\/v4_taxa_tab3Silvav132"
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            "mediaType": "text\/plain",
            "title": "dada2_output\/v4_taxa_tab3rdp18"
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            "title": "dada2_output\/v3_taxa_tab3Silvav132"
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            "title": "dada2_output\/v3_taxa_tab3rdp18"
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            "mediaType": "text\/plain",
            "title": "dada2_output\/v3_taxa_tab3Silvav138"
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            "mediaType": "text\/plain",
            "title": "dada2_output\/v4_taxa_tab3Silvav138"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-3092\/DataAnalysis.Rmd",
            "mediaType": "application\/octet-stream",
            "title": "DataAnalysis"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-3092\/ProcessingRawReads.Rmd",
            "mediaType": "application\/octet-stream",
            "title": "ProcessingRawReads"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-3092\/raw_reads.zip",
            "mediaType": "application\/zip",
            "title": "raw_reads"
        },
        {
            "downloadURL": "https:\/\/data.nist.gov\/od\/ds\/mds2-3092\/resources.zip",
            "format": "R markdown document.",
            "description": "R code for analysis and figure generation.",
            "mediaType": "application\/zip",
            "title": "resources"
        }
    ],
    "license": "https:\/\/www.nist.gov\/open\/license",
    "bureauCode": [
        "006:55"
    ],
    "modified": "2023-10-05 00:00:00",
    "publisher": {
        "@type": "org:Organization",
        "name": "National Institute of Standards and Technology"
    },
    "theme": [
        "Bioscience:Microbial measurements"
    ],
    "issued": "2023-11-16",
    "keyword": [
        "microbiome",
        "metagenomic sequencing",
        "full factorial",
        "design of experiments"
    ]
}