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Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor
Data provided by National Institute of Standards and Technology
This database gives hydrogen-deuterium exchange mass spectrometry (HDX-MS) data from measurements of three purified IgG1 glycoform samples, predominantly G0F, G2F, and SAF, in isolation and in complexation with the high-affinity receptor, Fc(gamma)RIa (CD64). The IgG1 antibody used in this study, aIL8hFc, is a murine-human chimeric IgG1, which inhibits IL-8 binding to human neutrophils.
Tags: antibody-receptor interaction,chromatography,HDX-MS,hydrogen-deuterium exchange,glycosylation,mass spectrometry,monoclonal antibody,precision,peptide,protein,proteolysis,proteomics,receptor.,
Modified: 2024-02-22
Views: 0
NIST DART-MS Forensics Database (is-CID)
Data provided by National Institute of Standards and Technology
The NIST DART-MS Forensics Database is an evaluated collection of in-source collisionally-induced dissociation (is-CID) mass spectra of compounds of interest to the forensics community (e.g. seized drugs, cutting agents, etc.). The is-CID mass spectra were collected using Direct Analysis in Real-Time (DART) Mass Spectrometry (MS), either by NIST scientists or by contributing agencies noted per compound. The database is provided as a general-purpose structure data file (.SDF).
Tags: Standard reference data,mass spectra,Ion Fragmentation,mass spectrometry,NIST Mass Spectral Libraries,Chemical Identification,Biosciences and Health,Security and Forensics,
Modified: 2024-02-22
Views: 0
NIST DART-MS Forensics Database (is-CID)
Data provided by National Institute of Standards and Technology
The NIST DART-MS Forensics Database is an evaluated collection of in-source collisionally-induced dissociation (is-CID) mass spectra of compounds of interest to the forensics community (e.g. seized drugs, cutting agents, etc.). The is-CID mass spectra were collected using Direct Analysis in Real-Time (DART) Mass Spectrometry (MS), either by NIST scientists or by contributing agencies noted per compound. The database is provided as a general-purpose structure data file (.SDF).
Tags: Standard reference data,mass spectra,Ion Fragmentation,mass spectrometry,NIST Mass Spectral Libraries,Chemical Identification,Biosciences and Health,Security and Forensics,
Modified: 2024-02-22
Views: 0
SRM 1450e Fibrous Glass Board
Data provided by National Institute of Standards and Technology
SRM 1450e Fibrous Glass Board - This Standard Reference Material (SRM) is intended primarily for use in the measurement of the thermal conductivity or thermal resistance of insulation materials. SRM 1450e is a high-density fibrous glass board certified for bulk density and thermal conductivity. The SRM can be used in conjunction with ASTM C177 or ASTM C518. A unit of SRM 1450e consists of a square panel of fibrous glass and phenolic binder molded into a semi-rigid board.
Tags: bulk density,certified reference material,fit,guarded hot plate,high density molded fibrous glass board,model,regression analysis,Standard Reference Material,thermal conductivity,thermal insulation,
Modified: 2024-02-22
Views: 0
Construction of a Dual-enzyme, Subzero (-30 degree C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry
Data provided by National Institute of Standards and Technology
This tutorial provides mechanical drawings, electrical schematics, parts lists, STL files for 3D-printed parts, IGS files, and instructions for automated machining necessary for construction of a dual-protease, subzero, liquid chromatography system for hydrogen-deuterium exchange mass spectrometry. Electrical schematics for construction of a multi-zone temperature controller that regulate to ±0.1 oC are also included in this tutorial.
Tags: liquid chromatography,hydrogen-deuterium exchange,mass spectrometry,precision,peptide,protein,proteolysis,proteomics,reference material,temperature control.,
Modified: 2024-02-22
Views: 0
Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Centroid Data measured between 3.6 degrees C and 25.4 degrees C for the Fab Fragment of NISTmAb
Data provided by National Institute of Standards and Technology
The spreadsheet file reported herein provides centroid data, descriptive of deuterium uptake, for the Fab Fragment of NISTmAb (PDB: 5K8A) reference material, as measured by the bottom-up hydrogen-deuterium exchange mass spectrometry (HDX-MS) method. The protein sample was incubated in deuterium-rich solutions under uniform pH and salt concentrations between 3.6 degrees C and 25.4 degrees C for seven intervals ranging (0 to 14,400) s plus a control sample that simulates a Fab Fragment immersed for infinite time in D2O.
Tags: hydrogen-deuterium exchange,hydrogen exchange,HDX-MS,interlaboratory comparison,mass spectrometry,peptide,precision,proteomics,reference material,repeatability,reproducibility,
Modified: 2024-02-22
Views: 0
SRM 131h Refined Cast Iron
Data provided by National Institute of Standards and Technology
Standard Reference Material 131h Refined Cast Iron - This Standard Reference Material (SRM) is intended primarily for use in validation of chemical and instrumental methods of analysis. It can be used to validate value assignment of in-house reference materials and, if necessary, to calibrate carbon/sulfur analyzers. A unit of SRM 131h consists of a bottle containing 100 g of chips. This data is public in the Certificate of Analysis for this material.
Tags: low alloy steel,cast iron,carbon,sulfur,Standard Reference Material,SRM,SRM 131h,combustion,infrared detection,manufacturing specification,manufacturing,
Modified: 2024-02-22
Views: 0
SRM 2718a Green Petroleum Coke
Data provided by National Institute of Standards and Technology
Standard Reference Material 2718a Green Petroleum Coke - This Standard Reference Material (SRM) is intended primarily for use in the calibration of apparatus and the evaluation of techniques used in the analysis of green (raw) petroleum coke and other materials of a similar matrix. It can be used to validate value assignment of in-house reference materials. A unit of SRM 2718a consists of 50 g of green petroleum coke ground to pass a 250 µm (60 mesh) sieve, homogenized, packaged in an amber glass bottle under an argon atmosphere, and then sealed in an aluminized bag.
Tags: SRM,Standard Reference Material,petroleum coke,sulfur,Energy,
Modified: 2024-02-22
Views: 0
SRM 2585 Organic Contaminants in House Dust
Data provided by National Institute of Standards and Technology
SRM 2585 Organic Contaminants in House Dust - This Standard Reference Material (SRM) is intended for use in evaluating analytical methods for the determination of selected polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyl (PCB) congeners, chlorinated pesticides, and polybrominated diphenyl ether (PBDE) congeners in house dust and similar matrices. All of the constituents for which certified, reference, and information values are provided in SRM 2585 were naturally present in the dust material before processing.
Tags: Standard Reference Material,SRM,SRM 2585,House Dust,certified values,Environment and Climate,
Modified: 2024-02-22
Views: 0
SRM 972a Vitamin D Metabolites in Frozen Human Serum
Data provided by National Institute of Standards and Technology
SRM 972a Vitamin D Metabolites in Frozen Human Serum - This Standard Reference Material (SRM) is intended for use as an accuracy control in the critical evaluation of methods for determining the amount-of-substance concentration of vitamin D metabolites in human serum. This SRM can also be used as a quality assurance tool for assigning values to in-house control materials for these constituents. This data is public in the Certificate of Analysis for this material.
Tags: Standard Reference Material,SRM,SRM 972a,Frozen Human Serum,certified values,reference values,Vitamin D Metabolites,25-hydroxyvitamin D3,25-hydroxyvitamin D2,3-epi-25-hydroxyvitamin D3,24R,25-dihydroxyvitamin D3,Biosciences and Health,
Modified: 2024-02-22
Views: 0