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77 results found

Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor

Data provided by  National Institute of Standards and Technology

This database gives hydrogen-deuterium exchange mass spectrometry (HDX-MS) data from measurements of three purified IgG1 glycoform samples, predominantly G0F, G2F, and SAF, in isolation and in complexation with the high-affinity receptor, Fc(gamma)RIa (CD64). The IgG1 antibody used in this study, aIL8hFc, is a murine-human chimeric IgG1, which inhibits IL-8 binding to human neutrophils.

Tags: antibody-receptor interaction,chromatography,HDX-MS,hydrogen-deuterium exchange,glycosylation,mass spectrometry,monoclonal antibody,precision,peptide,protein,proteolysis,proteomics,receptor.,

Modified: 2024-02-22

Views: 0

NIST DART-MS Forensics Database (is-CID)

Data provided by  National Institute of Standards and Technology

The NIST DART-MS Forensics Database is an evaluated collection of in-source collisionally-induced dissociation (is-CID) mass spectra of compounds of interest to the forensics community (e.g. seized drugs, cutting agents, etc.). The is-CID mass spectra were collected using Direct Analysis in Real-Time (DART) Mass Spectrometry (MS), either by NIST scientists or by contributing agencies noted per compound. The database is provided as a general-purpose structure data file (.SDF).

Tags: Standard reference data,mass spectra,Ion Fragmentation,mass spectrometry,NIST Mass Spectral Libraries,Chemical Identification,Biosciences and Health,Security and Forensics,

Modified: 2024-02-22

Views: 0

NIST DART-MS Forensics Database (is-CID)

Data provided by  National Institute of Standards and Technology

The NIST DART-MS Forensics Database is an evaluated collection of in-source collisionally-induced dissociation (is-CID) mass spectra of compounds of interest to the forensics community (e.g. seized drugs, cutting agents, etc.). The is-CID mass spectra were collected using Direct Analysis in Real-Time (DART) Mass Spectrometry (MS), either by NIST scientists or by contributing agencies noted per compound. The database is provided as a general-purpose structure data file (.SDF).

Tags: Standard reference data,mass spectra,Ion Fragmentation,mass spectrometry,NIST Mass Spectral Libraries,Chemical Identification,Biosciences and Health,Security and Forensics,

Modified: 2024-02-22

Views: 0

Construction of a Dual-enzyme, Subzero (-30 degree C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry

Data provided by  National Institute of Standards and Technology

This tutorial provides mechanical drawings, electrical schematics, parts lists, STL files for 3D-printed parts, IGS files, and instructions for automated machining necessary for construction of a dual-protease, subzero, liquid chromatography system for hydrogen-deuterium exchange mass spectrometry. Electrical schematics for construction of a multi-zone temperature controller that regulate to ±0.1 oC are also included in this tutorial.

Tags: liquid chromatography,hydrogen-deuterium exchange,mass spectrometry,precision,peptide,protein,proteolysis,proteomics,reference material,temperature control.,

Modified: 2024-02-22

Views: 0

Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Centroid Data measured between 3.6 degrees C and 25.4 degrees C for the Fab Fragment of NISTmAb

Data provided by  National Institute of Standards and Technology

The spreadsheet file reported herein provides centroid data, descriptive of deuterium uptake, for the Fab Fragment of NISTmAb (PDB: 5K8A) reference material, as measured by the bottom-up hydrogen-deuterium exchange mass spectrometry (HDX-MS) method. The protein sample was incubated in deuterium-rich solutions under uniform pH and salt concentrations between 3.6 degrees C and 25.4 degrees C for seven intervals ranging (0 to 14,400) s plus a control sample that simulates a Fab Fragment immersed for infinite time in D2O.

Tags: hydrogen-deuterium exchange,hydrogen exchange,HDX-MS,interlaboratory comparison,mass spectrometry,peptide,precision,proteomics,reference material,repeatability,reproducibility,

Modified: 2024-02-22

Views: 0

Certified values of iodine in CRMs of food matrices in mg/kg units. The uncertainties are expanded uncertainties at approximately 95% confidence.

Data provided by  National Institute of Standards and Technology

A comparison of the expanded uncertainty for certified values of iodine in food matrix CRMs. The CRMS were found by searching The European Virtual Institute for Speciation Analysis (EVISA) database using the terms "Iodine" and "Certified" in the Material category. The uncertainties are expanded uncertainties at approximately 95% confidence.

Tags: food,nutrition,dietary supplements,Standard Reference materials,reference materials,SRM,RM,Food and Nutrition,

Modified: 2024-02-22

Views: 0

SRM 131h Refined Cast Iron

Data provided by  National Institute of Standards and Technology

Standard Reference Material 131h Refined Cast Iron - This Standard Reference Material (SRM) is intended primarily for use in validation of chemical and instrumental methods of analysis. It can be used to validate value assignment of in-house reference materials and, if necessary, to calibrate carbon/sulfur analyzers. A unit of SRM 131h consists of a bottle containing 100 g of chips. This data is public in the Certificate of Analysis for this material.

Tags: low alloy steel,cast iron,carbon,sulfur,Standard Reference Material,SRM,SRM 131h,combustion,infrared detection,manufacturing specification,manufacturing,

Modified: 2024-02-22

Views: 0

SRM 2718a Green Petroleum Coke

Data provided by  National Institute of Standards and Technology

Standard Reference Material 2718a Green Petroleum Coke - This Standard Reference Material (SRM) is intended primarily for use in the calibration of apparatus and the evaluation of techniques used in the analysis of green (raw) petroleum coke and other materials of a similar matrix. It can be used to validate value assignment of in-house reference materials. A unit of SRM 2718a consists of 50 g of green petroleum coke ground to pass a 250 µm (60 mesh) sieve, homogenized, packaged in an amber glass bottle under an argon atmosphere, and then sealed in an aluminized bag.

Tags: SRM,Standard Reference Material,petroleum coke,sulfur,Energy,

Modified: 2024-02-22

Views: 0

SRM 1953 Organic Contaminants in Non-Fortified Human Milk

Data provided by  National Institute of Standards and Technology

SRM 1953 Organic Contaminants in Non-Fortified Human Milk - This Standard Reference Material (SRM) is intended for use in evaluating analytical methods for the determination of selected polychlorinated biphenyl (PCB) congeners, chlorinated pesticides, polybrominated diphenyl ether (PBDE) congeners, and elements in human milk and similar matrices. Reference concentration values are provided for polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs), as well as some inorganic constituents.

Tags: environment,Standard Reference materials,reference materials,SRM,RM,Environment and Climate,

Modified: 2024-02-22

Views: 0

SRM 2585 Organic Contaminants in House Dust

Data provided by  National Institute of Standards and Technology

SRM 2585 Organic Contaminants in House Dust - This Standard Reference Material (SRM) is intended for use in evaluating analytical methods for the determination of selected polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyl (PCB) congeners, chlorinated pesticides, and polybrominated diphenyl ether (PBDE) congeners in house dust and similar matrices. All of the constituents for which certified, reference, and information values are provided in SRM 2585 were naturally present in the dust material before processing.

Tags: Standard Reference Material,SRM,SRM 2585,House Dust,certified values,Environment and Climate,

Modified: 2024-02-22

Views: 0